推荐产品
生物来源
bovine fetus
质量水平
表单
frozen liquid
浓度
≥14 mg protein/mL Biuret
技术
cell culture | mammalian: suitable
cell culture | stem cell: suitable
杂质
<1 ng/mg protein endotoxin
<5 % of normal lipoprotein
运输
dry ice
储存温度
−20°C
一般描述
研究表明，使用脂蛋白缺乏血清条件下培养的人成纤维细胞中甾醇的产生减少。
应用
来自胎牛的脂蛋白缺乏血清已被用作培养基中的补充剂，用于培养中国仓鼠卵巢（CHO）衍生细胞、胸腺叶和人外周血单核细胞（PBMC）。
制备说明
无菌过滤， 0.2 μm
用 Dulbecco′s 磷酸盐缓冲盐水（pH 7.4-7.5）进行透析
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
法规信息
监管及禁止进口产品
Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Ying Wang et al.
Cell death discovery, 7(1), 201-201 (2021-08-06)
Coenzyme Q (CoQ) is a lipid-like mobile electron transporter of the mitochondrial respiratory chain. Patients with partial loss-of-function mutations in the CoQ biosynthesis pathway suffer from partial primary CoQ deficiency (MIM 607426). This leads to mitochondrial dysfunction, which presents like
Sterol regulatory element-binding protein (SREBP) cleavage regulates Golgi-to-endoplasmic reticulum recycling of SREBP cleavage-activating protein (SCAP)
Shao W and Espenshade PJ
The Journal of Biological Chemistry, 289(11), 7547-7557 (2014)
Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
实验方案
Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.
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