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主要文件

安全信息

S5394

Sigma-Aldrich

乏脂血清 来源于胎牛

frozen liquid

别名:

Fetal Calf Serum, Lipoprotein Deficient Serum

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.61
生物来源:
bovine fetus
表单:
frozen liquid
运输:
dry ice

生物来源

bovine fetus

质量水平

表单

frozen liquid

浓度

≥14 mg protein/mL Biuret

技术

cell culture | mammalian: suitable
cell culture | stem cell: suitable

杂质

<1 ng/mg protein endotoxin
<5 % of normal lipoprotein

运输

dry ice

储存温度

−20°C

一般描述

研究表明,使用脂蛋白缺乏血清条件下培养的人成纤维细胞中甾醇的产生减少。

应用

来自胎牛的脂蛋白缺乏血清已被用作培养基中的补充剂,用于培养中国仓鼠卵巢(CHO)衍生细胞、胸腺叶和人外周血单核细胞(PBMC)。

制备说明

无菌过滤, 0.2 μm
用 Dulbecco′s 磷酸盐缓冲盐水(pH 7.4-7.5)进行透析

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

监管及禁止进口产品

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分析证书(COA)

Lot/Batch Number

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Brian T Emmer et al.
PLoS genetics, 17(1), e1009285-e1009285 (2021-01-30)
Hypercholesterolemia is a causal and modifiable risk factor for atherosclerotic cardiovascular disease. A critical pathway regulating cholesterol homeostasis involves the receptor-mediated endocytosis of low-density lipoproteins into hepatocytes, mediated by the LDL receptor. We applied genome-scale CRISPR screening to query the
P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
Liliana M R Silva et al.
Frontiers in cellular and infection microbiology, 12, 809606-809606 (2022-03-01)
Apicomplexan parasites are considered as defective in cholesterol synthesis. Consequently, they need to scavenge cholesterol from the host cell by either enhancing the uptake of extracellular cholesterol sources or by upregulating host cellular de-novo biosynthesis. Given that Eimeria bovis macromeront
Ying Wang et al.
Cell death discovery, 7(1), 201-201 (2021-08-06)
Coenzyme Q (CoQ) is a lipid-like mobile electron transporter of the mitochondrial respiratory chain. Patients with partial loss-of-function mutations in the CoQ biosynthesis pathway suffer from partial primary CoQ deficiency (MIM 607426). This leads to mitochondrial dysfunction, which presents like
Ning Huangfu et al.
Frontiers in cell and developmental biology, 9, 687169-687169 (2021-07-23)
Atherosclerosis (AS), characterized by cholesterol overloaded-macrophages accumulation and plaque formation in blood vessels, is the major cause of cardiovascular disease. Transactive response DNA-binding protein∼43 kDa (TDP43) has recently been identified as an independent driver of neurodegenerative diseases through triggering inflammatory

实验方案

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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