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无菌性
sterile-filtered
质量水平
产品线
BioReagent
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
运输
ambient
储存温度
2-8°C
一般描述
胰岛素-转铁蛋白-硒-乙醇胺添加剂（100×SITE液体培养基补充剂）是适宜的无血清培养基替代品，含有适合体外生长和分化研究的纯净因子。补充剂在培养基中的添加量并不一定，取决于研究细胞类型和采用的基础培养基。它是一种通用的细胞补充剂，设计用于含有丙酮酸钠的非复合型培养基（如最低必需培养基(MEM)、Roswell Park Memorial Institute培养基(RPMI-1640)）及复合型培养基（如Ham′s F-12、杜氏改良Eagle培养基(DME)/F-12、MEM）。
应用
胰岛素-转铁蛋白-硒-乙醇胺添加剂（100×SITE液体培养基补充剂）已用于：
- 小鼠胚胎胰腺上皮细胞3D培养
- 兔胃腺和胃壁细胞分离
- 兔肾近端小管上皮细胞分离
生化/生理作用
- 胰岛素是一种多肽激素，可促进葡萄糖和氨基酸的摄取，这种特性可能与观察到的有丝分裂作用有关。
- 转铁蛋白：铁运输蛋白。铁是一种必需微量元素，但游离形式可能有毒。为了保证细胞培养的营养，可与血清转铁蛋白结合后使用。
- 硒：通常由血清提供的必需微量元素。
- 乙醇胺：对杂交瘤细胞增殖起重要作用，这类细胞的培养基补充剂中常添加乙醇胺。
组分
含有1.0mg/ml人重组胰岛素，0.55mg/ml 人转铁蛋白（基本上不含贴），0.5μg/ml 亚硒酸钠，以及 0.2mg/ml 乙醇胺，100x 浓缩液。
制备说明
使用不含酚红的Earle's平衡盐溶液（EBSS）制备。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Lixin Zhu et al.
American journal of physiology. Cell physiology, 295(1), C192-C202 (2008-05-16)
In a comparison of three different tissues, the membrane cytoskeleton linker protein ezrin was found to assume high levels of phosphorylation on threonine-567 (T567) in the brush border membranes of renal proximal tubule cells and small intestine enterocytes, in contrast
H Murakami et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(4), 1158-1162 (1982-02-01)
A serum-free medium supplemented with a few growth factors was devised to grow lymphocyte hybridomas. The medium was developed with the hybridoma line MPC11-BL, a fusion product between a mouse plasmacytoma cell line (MPC11TG70na3) and mouse (BALB/c) spleen cells. In
Lixin Zhu et al.
American journal of physiology. Cell physiology, 293(3), C874-C884 (2007-06-08)
In its dormant state, the membrane cytoskeletal linker protein ezrin takes on a NH(2) terminal-to-COOH terminal (N-C) binding conformation. In vitro evidence suggests that eliminating the N-C binding conformation by Thr(567) phosphorylation leads to ezrin activation. Here, we found for
Nico Laur et al.
PloS one, 15(5), e0233357-e0233357 (2020-05-21)
Trace elements and minerals are compounds that are essential for the support of a variety of biological functions and play an important role in the formation of and the defense against oxidative stress. Here we describe a technique, allowing sequential
Gisela Wilcox
The Clinical biochemist. Reviews, 26(2), 19-39 (2005-11-10)
As obesity and diabetes reach epidemic proportions in the developed world, the role of insulin resistance and its consequences are gaining prominence. Understanding the role of insulin in wide-ranging physiological processes and the influences on its synthesis and secretion, alongside
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