一般描述
胰岛素-转铁蛋白-硒-乙醇胺添加剂（100×SITE液体培养基补充剂）是适宜的无血清培养基替代品，含有适合体外生长和分化研究的纯净因子。补充剂在培养基中的添加量并不一定，取决于研究细胞类型和采用的基础培养基。它是一种通用的细胞补充剂，设计用于含有丙酮酸钠的非复合型培养基（如最低必需培养基(MEM)、Roswell Park Memorial Institute培养基(RPMI-1640)）及复合型培养基（如Ham′s F-12、杜氏改良Eagle培养基(DME)/F-12、MEM）。
应用
胰岛素-转铁蛋白-硒-乙醇胺添加剂（100×SITE液体培养基补充剂）已用于：
- 小鼠胚胎胰腺上皮细胞3D培养
- 兔胃腺和胃壁细胞分离
- 兔肾近端小管上皮细胞分离
生化/生理作用
- 胰岛素是一种多肽激素，可促进葡萄糖和氨基酸的摄取，这种特性可能与观察到的有丝分裂作用有关。
- 转铁蛋白：铁运输蛋白。铁是一种必需微量元素，但游离形式可能有毒。为了保证细胞培养的营养，可与血清转铁蛋白结合后使用。
- 硒：通常由血清提供的必需微量元素。
- 乙醇胺：对杂交瘤细胞增殖起重要作用，这类细胞的培养基补充剂中常添加乙醇胺。
组分
含有1.0mg/ml人重组胰岛素，0.55mg/ml 人转铁蛋白（基本上不含贴），0.5μg/ml 亚硒酸钠，以及 0.2mg/ml 乙醇胺，100x 浓缩液。
制备说明
使用不含酚红的Earle's平衡盐溶液（EBSS）制备。
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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