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安全信息

S4762

Sigma-Aldrich

链亲和素 来源于阿维丁链霉菌

essentially salt-free, lyophilized powder, ≥13 units/mg protein

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About This Item

CAS号:
9013-20-1
MDL编号:
MFCD00082035
UNSPSC代码:
12352203
NACRES:
NA.46

形式

essentially salt-free, lyophilized powder

质量水平

200

比活

≥13 units/mg protein

分子量

~60 kDa

容量

4 mol/mol (Biotin)

应用

research pathology

储存温度

−20°C

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相关类别

一般描述

链霉亲和素得名于它的细菌来源亲和素链霉菌 和母鸡蛋清蛋白亲和素，而后者与生物素有很高的亲和力。其同源核与亲和素具有33%的序列相似性，并具有共同的四聚体结构。这是一种晶体四聚体蛋白，分子量为4*15000Da。它可结合四种生物素分子。链霉亲和素缺乏碳水化合物和含硫氨基酸。
链霉亲和素是从亲和素链霉菌中分离得到的一种结晶蛋白，具有生物素结合能力。它有四个生物素（如亲和素）结合位点并可结合四个生物素分子。链霉亲和素现被视为一种新型的生物素结合蛋白。

应用

来自抗生物素蛋白链霉菌的链霉亲和素已用于：
  • 的线性官能化
  • 链霉亲和素捕获酶联免疫吸附测定（ELISA）
  • 微泡制备

制备说明

亲和纯化。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物应用。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. In what is Product No. S4762 soluble? And how do you recommend storing solutions of streptavidin?

    This product is soluble in water or aqueous buffer (i.e., PBS) at 1 mg/mL. Solutions in PBS or water can be stored for several months at 4°C, or for more than a year as frozen aliquots.

  6. How do I determine how many units are in a particular amount of Product No. S4762?

    On the Certificate of Analysis for the product, the activity of the product per milligram can be found. This number is specific for your lot, and you must look up the Certificate of Analysis by the lot number for your particular batch of product. From this number, you can calculate how many units are in a particular weight of your product by multiplying the weight of the product by the units/mg of the product.

  7. What is the expiration date of the Product No. S4762 in my possession?

    Product No. S4762 has not been assigned an expiration date by Sigma. Streptavidin is stable. S4762 has been assigned a QC Release date as well was a Retest Date. These dates can be found on the Certificate of Analysis. The QC Release Date indicates the last date that the lot was tested and found to meet specifications, and the Retest Date indicates the next date that the lot will be tested, assuming that enough of the lot remains to perform the testing.

  8. What is the affinity of streptavidin (e.g., Product No. S4762) for biotin?

    Streptavidin binds the small molecule biotin with extraordinary affinity. The dissociation constant is KD = 4 × 10-14 M, according to N. M. Green, Methods Enzymol., 184, 51 (1990).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Viability of endothelial cells after ultrasound-mediated sonoporation: Influence of targeting, oscillation, and displacement of microbubbles
van R T, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 238, 197-211 (2016)
Isolation and properties of streptavidin.
E A Bayer et al.
Methods in enzymology, 184, 80-89 (1990-01-01)
Biotinylated CdSe/ZnSe nanocrystals for specific fluorescent labeling.
Charvet N et al
Journal of Materials Chemistry, 14.17 , 2638-2642 (2004)
A rapid streptavidin-capture ELISA specific for the detection of antibodies to feline foamy virus.
Winkler IG et al
Journal of Immunological Methods, 207(1), 69-77 (1997)
Mapping of functional domains in the p22(phox) subunit of flavocytochrome b(559) participating in the assembly of the NADPH oxidase complex by "peptide walking".
Dahan I et al
The Journal of Biological Chemistry, 277(10), 8421-8432 (2002)
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