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安全信息

S4760

Sigma-Aldrich

N-琥珀酰-丙氨酸-丙氨酸-丙氨酸-p-硝基苯胺

elastase substrate

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别名:
N-琥珀酰-L-丙氨酰-L-丙氨酰-L-丙氨酸4-硝基苯胺, N-琥珀酰-三-L-丙氨酸4-硝基苯胺
经验公式（希尔记法）:
C19H25N5O8
CAS号:
52299-14-6
分子量:
451.43
Beilstein:
2928351
EC 号:
257-823-5
MDL编号:
MFCD00036774
UNSPSC代码:
12352204
PubChem化学物质编号:
24899617
NACRES:
NA.32

质量水平

200

检测方案

≥98% (HPLC)

形式

powder

溶解性

DMF: 25 mg/mL, clear, colorless to light yellow

储存温度

2-8°C

SMILES字符串

C[C@H](NC(=O)CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)Nc1ccc(cc1)[N+]([O-])=O

InChI

1S/C19H25N5O8/c1-10(20-15(25)8-9-16(26)27)17(28)21-11(2)18(29)22-12(3)19(30)23-13-4-6-14(7-5-13)24(31)32/h4-7,10-12H,8-9H2,1-3H3,(H,20,25)(H,21,28)(H,22,29)(H,23,30)(H,26,27)/t10-,11-,12-/m0/s1

InChI key

GVUGADOWXGKRAE-SRVKXCTJSA-N

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一般描述

N-琥珀酰-Ala-Ala-Ala-对硝基苯胺是糜蛋白酶样丝氨酸蛋白酶、胰弹性蛋白酶和丝氨酸内肽酶的显色底物。

应用

N-琥珀酰-Ala-Ala-Ala-对硝基苯胺作为底物用于
  • 筛选冻干蚯蚓纤溶酶中弹性蛋白酶的活性
  • 量化羊奶中的弹性蛋白酶的活性
  • 在丝氨酸蛋白酶抑制分析中筛选蜜蜂抑制剂

底物

弹性蛋白酶底物。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

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Transgenic chickpea expressing a recombinant human a1-proteinase inhibitor (a1-PI) driven by a seed-specific promoters from the common bean Phaseolus vulgaris (L.).
Mishra, S., Jha, S., Singh, R., Chaudhary, S., Sanyal, I., & Amla, D. V.
Plant Cell, Tissue and Organ Culture, 115, 23-33 (2013)
V J Uitto et al.
Journal of clinical periodontology, 23(1), 30-37 (1996-01-01)
The study was designed to find out whether oral elastase activity could be used as a simple biochemical indicator of periodontal health. Both stimulated whole saliva and water rinse samples were collected from subjects with different degrees of adult periodontitis
In Situ and Multisubstrate Detection of Elastase Enzymatic Activity External to Microdialysis Sampling Probes Using LC- ESI-MS
Wang Y, et al.
Analytical Chemistry, 80(6), 2050-2057 (2008)
M Rouis et al.
Arteriosclerosis (Dallas, Tex.), 10(2), 246-255 (1990-03-01)
We investigated the effects of a number of stimulatory agents on the production of both cell-associated and extracellular elastase-type enzymes on human monocyte-macrophages in vitro and of the modulation of such effects by modification of cellular cholesterol content. The stimulatory
Ulvi Kahraman Gürsoy et al.
Pathogens (Basel, Switzerland), 9(9) (2020-09-19)
Aim was to profile salivary total protease, Porphyromonas gingivalis gingipain, and neutrophil elastase activities in relation to the resolution of periodontal inflammation, salivary macrophage-derived chemokine (MDC), and macrophage inflammatory protein-1α concentrations. Nonsurgical periodontal treatment was performed in 24 periodontitis patients
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Elastase Function and Importance

Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.

实验方案

Enzymatic Assay of Elastase (EC 3.4.21.36)

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

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