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Merck
CN

S4760

N-琥珀酰-丙氨酸-丙氨酸-丙氨酸-p-硝基苯胺

elastase substrate, chromogenic, ≥98% (HPLC), powder

别名:

N-琥珀酰-L-丙氨酰-L-丙氨酰-L-丙氨酸4-硝基苯胺, N-琥珀酰-三-L-丙氨酸4-硝基苯胺

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关于此项目

经验公式（希尔记法）:
C19H25N5O8
化学文摘社编号:
52299-14-6
分子量:
451.43
NACRES:
NA.32
PubChem Substance ID:
24899617
UNSPSC Code:
12352204
EC Number:
257-823-5
MDL number:
MFCD00036774
Beilstein/REAXYS Number:
2928351

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产品名称

N-琥珀酰-丙氨酸-丙氨酸-丙氨酸-p-硝基苯胺, elastase substrate

InChI key

GVUGADOWXGKRAE-SRVKXCTJSA-N

InChI

1S/C19H25N5O8/c1-10(20-15(25)8-9-16(26)27)17(28)21-11(2)18(29)22-12(3)19(30)23-13-4-6-14(7-5-13)24(31)32/h4-7,10-12H,8-9H2,1-3H3,(H,20,25)(H,21,28)(H,22,29)(H,23,30)(H,26,27)/t10-,11-,12-/m0/s1

SMILES string

C[C@H](NC(=O)CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)Nc1ccc(cc1)[N+]([O-])=O

assay

≥98% (HPLC)

form

powder

solubility

DMF: 25 mg/mL, clear, colorless to light yellow

storage temp.

2-8°C

Quality Level

200

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Application

N-琥珀酰-Ala-Ala-Ala-对硝基苯胺作为底物用于
  • 筛选冻干蚯蚓纤溶酶中弹性蛋白酶的活性
  • 量化羊奶中的弹性蛋白酶的活性
  • 在丝氨酸蛋白酶抑制分析中筛选蜜蜂抑制剂

General description

N-琥珀酰-Ala-Ala-Ala-对硝基苯胺是糜蛋白酶样丝氨酸蛋白酶、胰弹性蛋白酶和丝氨酸内肽酶的显色底物。
弹性蛋白酶底物。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000521131
0000521133
0000521132
0000521132
0000521133

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Role of endogenous enzymes in proteolysis of sheep milk
Albenzio M, et al.
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SEP-1-a subtilisin-like serine endopeptidase from germinated seeds of Hordeum vulgare L. cv. Morex
Fontanini D and Jones BL
Planta, 215(6), 885-893 (2002)
Transgenic chickpea expressing a recombinant human a1-proteinase inhibitor (a1-PI) driven by a seed-specific promoters from the common bean Phaseolus vulgaris (L.).
Mishra, S., Jha, S., Singh, R., Chaudhary, S., Sanyal, I., & Amla, D. V.
Plant Cell, Tissue and Organ Culture, 115, 23-33 (2013)
Ulvi Kahraman Gürsoy et al.
Pathogens (Basel, Switzerland), 9(9) (2020-09-19)
Aim was to profile salivary total protease, Porphyromonas gingivalis gingipain, and neutrophil elastase activities in relation to the resolution of periodontal inflammation, salivary macrophage-derived chemokine (MDC), and macrophage inflammatory protein-1α concentrations. Nonsurgical periodontal treatment was performed in 24 periodontitis patients
M Rouis et al.
Arteriosclerosis (Dallas, Tex.), 10(2), 246-255 (1990-03-01)
We investigated the effects of a number of stimulatory agents on the production of both cell-associated and extracellular elastase-type enzymes on human monocyte-macrophages in vitro and of the modulation of such effects by modification of cellular cholesterol content. The stimulatory
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商品

Elastase Function and Importance

Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.

实验方案

Enzymatic Assay of Elastase (EC 3.4.21.36)

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

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