推荐产品
产品名称
Sephacryl®, 400-HR, MW range 20-8000 kDa (globular proteins)
质量水平
描述
stability: stable to 0.2M NaOH
表单
suspension (20% aqueous ethanol)
特点
autoclavable
基质
crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
粒径
25-75 μm (wet)
孔径
10-2000 kDa MW range (dextrans)
20-8000 kDa MW range (globular proteins)
相容性
mode of use size exclusion chromatography
储存温度
2-8°C
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一般描述
- 肿胀，悬浮在 20% 乙醇水溶液中
- 高压灭菌
- 在 0.2 N 氢氧化钠 (NaOH) 中稳定
- 冷藏温度 (2-8°C) 下储存
S400HR-750 mL的更新后产品为 GE17-0609-01
应用
用于分子排阻色谱；HR级是较小的粒径，具有较窄的大小范围分布，能够以更快的流速实现更有效的分离。 已列出了球状蛋白质和葡聚糖的推荐分馏范围。
聚丙烯酰胺葡聚糖凝胶® 用于凝胶过滤色谱、 蛋白色谱以及树脂和分离介质。聚丙烯酰胺葡聚糖凝胶® 已被用于开发一种新的高通量筛选分析，用于镰状细胞病药物发现。聚丙烯酰胺葡聚糖凝胶® 还用于纯化和表征酶，如牛胰弹性蛋白酶、组织非特异性碱性磷酸酶 和来自人尿液的溶菌酶。
法律信息
Sephacryl is a registered trademark of Cytiva
警示用语：
Warning
危险声明
危险分类
Flam. Liq. 3
储存分类代码
3 - Flammable liquids
WGK
WGK 1
闪点(°F)
100.4 - 109.4 °F - closed cup
闪点(°C)
38 - 43 °C - closed cup
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
危险化学品
E Käslan et al.
The Journal of biological chemistry, 269(19), 14103-14110 (1994-05-13)
During purification of a strand exchange activity from Schizosaccharomyces pombe using the three-strand reaction of double-stranded linear and circular single-stranded DNA, we identified p190/210 as an activity that stimulated the strand exchange activity of p140exo2 by about 10-fold. The accompanying
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Hydrogen peroxide (H2O2) is an important signal molecule produced in animal and plant cells. The balance of H2O2 between the intra- and extracellular space is regulated by integral membrane proteins, which thereby modulate signaling. Several methods have been established to
Enhancement of drug delivery to bone: Characterization of human tissue-nonspecific alkaline phosphatase tagged with an acidic oligopeptide
Nishioka T, et al.
Molecular Genetics and Metabolism, 88(3), 244-255 (2006)
Michael J Lodes et al.
Methods in molecular medicine, 94, 91-106 (2004-02-13)
Expression cloning involves the selection of specific polypeptides, generated from a cDNA or genomic DNA library, based on certain characteristics of the expressed proteins, such as antibody or ligand binding, recognition by T-cells, function, or complementation of cell defects. Here
Masaharu Tsuji et al.
International journal of systematic and evolutionary microbiology, 69(4), 944-948 (2019-01-04)
Four strains isolated from sediment sampled at the front of a retreating glacier on northern Ellesmere Island in the Canadian high Arctic, namely JCM 32575T, JCM 32576, JCM 32577 and JCM 32578, belong to a novel psychrophilic basidiomycetous yeast species
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