产品名称
Monoclonal Anti-Spectrin (α and β) antibody produced in mouse, clone SB-SP1, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
SB-SP1, monoclonal
contains
15 mM sodium azide
species reactivity
human
technique(s)
indirect immunofluorescence: suitable using erythrocyte smears
microarray: suitable
western blot: 1:500 using extract of human erythrocyte ghosts or purified human erythrocyte spectrin
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SPTA1(6708), SPTB(6710)
Application
Anti-Spectrin (α and β) antibody has been used in western blotting and immunostaining.
Monoclonal Anti-Spectrin (α and β) antibody produced in mouse has been used in flow cytometry analysis and confocal microscopy.
Biochem/physiol Actions
Spectrins are multifunctional proteins, that modulates the cell morphology and mechanical properties. Spectrins also participates in the cell cycle by controlling the upregulation of membrane receptors. α- and β-spectrins are essential for the development of nervous system.
This antibody reacts specifically with the α and β chains of human erythrocyte spectrin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal anti-Spectrin (α and β) (mouse IgG1 isotype) is produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass.
Spectrins are heterotetramers, that are composed of several αand β subunit isoforms. They are the constituents of G-protein-coupled receptor (GPCR) and the synaptic multiprotein complex. α-Spectrin has 20 spectrin repeats (α1-α20) and β-spectrin has 17 spectrin repeats (β1-β17). SPTA1 (spectrin α 1) is located on chromosome 1q21–q23 and SPTB (spectrin β) is located on chromosome 14q22–q23.2.
Immunogen
human erythrocyte spectrin.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Membrane protein carbonylation in non-leukodepleted CPDA-preserved red blood cells
Kriebardis AG, et al.
Blood Cells, Molecules and Diseases, 36(2), 279-282 (2006)
Julie D Atkin et al.
The Journal of biological chemistry, 281(40), 30152-30165 (2006-07-19)
Mutations in Cu/Zn superoxide dismutase (SOD1) are linked to motor neuron death in familial amyotrophic lateral sclerosis (ALS) by an unclear mechanism, although misfolded SOD1 aggregates are commonly associated with disease. Proteomic analysis of the transgenic SOD1(G93A) ALS rat model
Indicators of erythrocyte damage after microwave warming of packed red blood cells
Hirsch J, et al.
Clinical Chemistry, 49(5), 792-799 (2003)
Specificity of commercial anti-spectrin antibody in the study of fungal and Oomycete spectrin: cross-reaction with proteins other than spectrin
Cotado-Sampayo M, et al.
Fungal genetics and biology : FG & B, 45(6), 1008-1015 (2008)
Spectrin labeling during oogenesis in zebrafish (Danio rerio)
Okuthe GE and Fabian BC
Acta Histochemica, 114(2), 177-181 (2012)
相关内容
Datasheet
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