S2657
Anti-S-100 (β-Subunit) antibody, Mouse monoclonal
clone SH-B4, purified from hybridoma cell culture
别名:
Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse, Anti-NEF, Anti-S100-B
产品名称
Anti-S-100 (β-Subunit) antibody, Mouse monoclonal, clone SH-B4, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
(Solution in 0.01M phosphate buffered saline pH 7.4, containing 15mM sodium azide as a preservative)
antibody product type
primary antibodies
clone
SH-B4, monoclonal
contains
15 mM sodium azide
species reactivity
sheep, bovine, goat, pig, feline, rabbit, rat, canine, human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable (: suitable)
indirect ELISA: suitable (:suitable)
western blot: 1:100-1:200 ( (using S-100B Protein, Bovine Brain))
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... S100B(6285)
rat ... S100b(25742)
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunocytochemistry (1 paper)
Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse has been used in immunocytochemistry and immunohistochemistry. It has also been used as a component in serum medium for 5-ethynyl-29-deoxyuridine (EdU), incorporation assay.
Biochem/physiol Actions
Recognizes an epitope located on the β chain (i.e. in S-100a and S-100b) but not on the α chain of S-100 (i.e. in S-100a and S-100ao). In ELISA, recognition of S-100 subunit by clone SH-B4 is Ca2+-dependent. The product does not react with other members of the EF-hand family such as, calmodulin, parvalbumin, intestinal calcium-binding protein and myosin light chain. In immunohistochemistry, the antibody detects normal and neoplastic S-100 β subunit-containing cells (e.g. Schwann cells, chondrocytes, melanocytes, and melanotic tumors) in protease-digested, formalin-fixed, paraffin-embedded tissues.
S-100 (β-Subunit) acts as a marker of melanocyte cytotoxicity. Reduced expression of S100B is observed in chronic liver diseases. It also acts as a serum marker in endocrine resistant breast cancer.
S-100 is considered to be a cell-growth regulator, but other functions have been suggested, e.g., increasing the membrane permeability to cations under physiologic conditions, stimulation of nucleolar RNA polymerase activity, interaction with the tumor suppressor protein p53 and as a carrier of proteins and free fatty acids in adipocytes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-S-100 (b-Subunit) (mouse IgG1 isotype) is derived from the SH-B4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. S-100 is a set of small, highly acidic, thermolabile dimer proteins of ~20 kDa, widely distributed in different tissues.
S-100 (β-Subunit) is a calcium-binding protein, that belongs to S100 family of calcium-binding proteins. It is expressed in melanocytes. It is located on human chromosome 21q22.3.
Immunogen
bovine brain S-100b
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
常规特殊物品
此项目有
S100B promotes injury-induced vascular remodeling through modulating smooth muscle phenotype
Cao T, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1863(11), 2772-2782 (2017)
Marked innervation but also signs of nerve degeneration in between the Achilles and plantaris tendons and presence of innervation within the plantaris tendon in midportion Achilles tendinopathy
Spang C, et al.
Journal of musculoskeletal & neuronal interactions, 15(2), 197-197 (2015)
Lingyan Fang et al.
Frontiers in neuroscience, 17, 1149603-1149603 (2023-07-17)
Adult neural stem cells (NSCs) in the mouse subventricular zone (SVZ) serve as a lifelong reservoir for newborn olfactory bulb neurons. Recent studies have identified a slowly dividing subpopulation of embryonic neural stem-progenitor cells (NPCs) as the embryonic origin of
S100 Proteins As an Important Regulator of Macrophage Inflammation
Xia C, et al.
Frontiers in Immunology (2017)
C Spang et al.
Journal of musculoskeletal & neuronal interactions, 15(2), 197-206 (2015-06-03)
The plantaris tendon is increasingly recognised as an important factor in midportion Achilles tendinopathy. Its innervation pattern is completely unknown. Plantaris tendons (n=56) and associated peritendinous tissue from 46 patients with midportion Achilles tendinopathy and where the plantaris tendon was
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