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质量水平
检测方案
≥98% (TLC)
形式
powder
溶解性
dioxane: 25 mg/mL, clear, colorless to faintly yellow
储存温度
−20°C
SMILES字符串
OC(=O)CCC(=O)N[C@@H](Cc1ccccc1)C(=O)Nc2ccc(cc2)N(=O)=O
InChI
1S/C19H19N3O6/c23-17(10-11-18(24)25)21-16(12-13-4-2-1-3-5-13)19(26)20-14-6-8-15(9-7-14)22(27)28/h1-9,16H,10-12H2,(H,20,26)(H,21,23)(H,24,25)/t16-/m0/s1
InChI key
KNBLWBFJHZHYFG-INIZCTEOSA-N
应用
N -琥珀酰- L -苯丙氨酸- p -硝基苯胺已被用作糜蛋白酶水解活性的底物和摩尔吸光实验。也用于体外监测 α-糜蛋白酶抑制试验。
生化/生理作用
糜蛋白酶水解活性的底物 N -琥珀酰- L -苯丙氨酸- p -硝基苯胺形成黄色生色团 p -硝基苯胺，可在 410 nm 处分光光度法测定。
底物
据报道，这是一种糜蛋白酶的底物。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
E Iu Maksareva et al.
Bioorganicheskaia khimiia, 21(1), 24-27 (1995-01-01)
Subtilisin Carlsberg (E.C. 3.4.21.14) catalyzes the hydrolysis of N-succinyl-L-phenylalanine p-nitroanilide in solid-state solvent-free hydrated protein-substrate mixtures. This process needs a certain critical degree of hydration of the protein molecule which is attained at the relative water vapour pressure (p/ps) above
Isabel Pastor et al.
The journal of physical chemistry. B, 115(5), 1115-1121 (2010-12-31)
Traditionally, studies on the diffusion-controlled reaction of biological macromolecules have been carried out in dilute solutions (in vitro). However, in an intracellular environment (in vivo), there is a high concentration of macromolecules, which results in nonspecific interactions (macromolecular crowding). This
Cyclopeptide alkaloids of Ziziphus oxyphylla Edgw as novel inhibitors of alpha-glucosidase enzyme and protein glycation
Choudhary MI, et al.
Phytochemistry Letters, 4(4), 404-406 (2011)
K Ashizawa et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 121(1), 77-83 (1999-02-11)
We investigated the effects of various protease substrates on the motility of demembranated fowl spermatozoa. In the presence of ATP, the motility of demembranated spermatozoa was vigorous at 30 degrees C, but decreased markedly following the addition of protease substrates
Mercury (II) binds to both of chymotrypsin's histidines, causing inhibition followed by irreversible denaturation/aggregation
Stratton A, et al.
Protein Science, 26(2), 292-305 (2017)
商品
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
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