形式
solution
质量水平
浓度
2 M
技术
protein expression: suitable
颜色
clear colorless
应用
life science and biopharma
储存温度
room temp
一般描述
Seppro®碳酸氢铵缓冲液是蛋白质消化和去糖基化反应的有效缓冲液。它可用于在蛋白质和蛋白质组的消化过程中包被 2D 和 3D 电泳凝胶片。碳酸氢铵缓冲液还在酶裂解过程中提供水分。碳酸氢铵缓冲液可调节固定化头孢菌素 C 酰基转移酶（CCA）的微环境 pH 值，用于在间歇搅拌釜式反应器（STR）中催化头孢菌素 C（CPC）。
应用
碳酸氢铵缓冲液已用作细胞裂解/蛋白质提取缓冲液的成分，用于制备蛋白质组样品。
法律信息
6538 is a trademark of American Type Culture Collection
Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Vimal K Balasubramanian et al.
Current protocols, 1(5), e153-e153 (2021-05-28)
Plant organs and tissues contain multiple cell types, which are well organized in 3-dimensional structure to efficiently perform physiological functions such as homeostasis and response to environmental perturbation and pathogen infection. It is critically important to perform molecular measurements at
Andrej Shevchenko et al.
Nature protocols, 1(6), 2856-2860 (2007-04-05)
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel
Yanhong Chang et al.
Biotechnology progress, 35(5), e2846-e2846 (2019-05-18)
In a stirred tank reactor, during catalysis with immobilized cephalosporin C acylase (CCA), the microenvironmental pH dropped to 7.2 in a nonbuffered system (with the pH maintained at 8.5 by adding alkali) due to the existence of diffusional resistance. Moreover
Emily S Boja et al.
The Journal of biological chemistry, 278(36), 34189-34202 (2003-06-12)
The zona pellucida is an extracellular matrix consisting of three glycoproteins that surrounds mammalian eggs and mediates fertilization. The primary structures of mouse ZP1, ZP2, and ZP3 have been deduced from cDNA. Each has a predicted signal peptide and a
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