一般描述
人ST6Gal-1（β半乳糖苷α-2,6-唾液酸转移酶1）是CAZy家族GT29的成员。
应用
α来自美人鱼发光杆菌（Photobacterium damsela） 的-2,6-唾液酸转移酶已被用于HRT-18G细胞中唾液酸（SA）的再唾液酸化（resialylation）和恢复。
高活性α 2-6唾液酸转移酶已用于制备高级别的的二唾液酸化片段晶体。
生化/生理作用
α复杂的N-聚糖生物合成的最终步骤是由-2,6-唾液酸转移酶（ST）催化。与真核生物α（2,6）-ST相比，细菌α（2,6）-ST具有更广泛的受体底物特异性。
唾液酸转移酶可将Neu5Ac从CMP-Neu5Ac转移至受体分子（包括糖蛋白、糖脂和寡糖）的半乳糖基末端。
单位定义
在37℃，pH8.0条件下，一个单位每分钟可催化由CMP-Neu-5-Ac和Lac-β-OMU形成1 μmol Neu-5-Ac-α -2,6-LacMU。
外形
以含有Tris-HCl和NaCl的冻干粉末形式提供。
分析说明
酶活性测定在含有CMP-Neu-5-Ac（1 mM）和Lac-β−OMU（1 mM）的Tris-HCl缓冲液（100 mM，pH 8.0）中于37°C进行30分钟，并使用带有荧光检测器（激发波长325nm，发射波长372nm）的HPLC进行分析。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Chompunuch Boonarkart et al.
Journal of medical virology, 84(3), 380-385 (2012-01-17)
A case of unusually high severity of influenza pneumonia leading to acute respiratory distress syndrome and death was investigated. This was a previously a healthy 28-year-old man with no underlying conditions, admitted to a hospital during the first wave of
Masayoshi Onitsuka et al.
Applied microbiology and biotechnology, 94(1), 69-80 (2011-12-30)
Improvement of glycosylation is one of the most important topics in the industrial production of therapeutic antibodies. We have focused on terminal sialylation with alpha-2,6 linkage, which is crucial for anti-inflammatory activity. In the present study, we have successfully cloned
N Li et al.
Acta virologica, 55(2), 147-153 (2011-06-23)
Human influenza viruses are major concern as the leading cause of global pandemics. In infecting cells, they preferentially bind to sialyloligosaccharides containing terminal N-acetyl sialic acid linked to galactose by an α-2,6-linkage (NeuAcα2,6Gal). The amount of NeuAcα2,6Gal in Vero cells
Miyako Nakano et al.
Molecular & cellular proteomics : MCP, 10(11), M111-M111 (2011-08-24)
Resistance to tubulin-binding agents used in cancer is often multifactorial and can include changes in drug accumulation and modified expression of tubulin isotypes. Glycans on cell membrane proteins play important roles in many cellular processes such as recognition and apoptosis
Enhanced Bacterial alpha (2, 6)-Sialyltransferase Reaction through an Inhibition of Its Inherent Sialidase Activity by Dephosphorylation of Cytidine-5'-Monophosphate
Kang JY, et al.
PLoS ONE, 10(7), e0133739-e0133739 (2015)
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