S1951
α-2,3-唾液酸转移酶 来源于多杀巴斯德菌
recombinant, expressed in E. coli BL21, ≥2 units/mg protein
别名:
CMP-N-乙酰神经氨酸酯:β-D-半乳糖苷α-(2,3)-N-乙酰神经氨酰转移酶
产品名称
α-2,3-唾液酸转移酶 来源于多杀巴斯德菌, recombinant, expressed in E. coli BL21, ≥2 units/mg protein
recombinant
expressed in E. coli BL21
form
lyophilized powder
specific activity
≥2 units/mg protein
mol wt
46.4 kDa
shipped in
dry ice
storage temp.
−20°C
Quality Level
Analysis Note
酶活性检测是在37°C条件下含有CMP-Neu-5Ac (1 mM)以及Lac-β−OMU (1 mM) 的Tris-HCl缓冲液(100 mM, pH 8.0)中反应30 min并通过使用带有荧光检测器（325 nm 激发而372 nm 发射）的HPLC而进行的。
Biochem/physiol Actions
唾液酸转移酶可将Neu5Ac从CMP-Neu5Ac作为一种供体底物转移至包括糖蛋白、糖脂和寡糖在内的受体分子的β-D-半乳糖基-1,4-N-乙酰基-D-氨基葡萄糖末端。
唾液酸转移酶可将Neu5Ac从CMP-Neu5Ac转移至受体分子（包括糖蛋白、糖脂和寡糖）的半乳糖基末端。
General description
唾液酸转移酶属于糖基转移酶家族，具有催化N-乙酰神经氨酸残基转移的能力。 它是一种具有α--2,3-唾液酸转移酶活性、α-2,6-唾液酸转移酶活性、唾液酸酶活性和反式唾液酸酶活性的多功能酶。 它的分子量大小为46.4 kDa，而pI和pH分别为5.94和7.5-8.5。
Other Notes
在37 °C pH 8.0条件下，一单位将会在没分钟内催化1.0 μmol Neu-5-Ac-α-2,3LacMU从CMP-Neu-5-Ac和Lac-β−OMU的形成。
Physical form
含有Tris-HCl和NaCl的冻干粉
Preparation Note
以0.1毫升至1毫升体积范围内的水调配冻干粉，可以得到1单位/毫升（1毫升水）至10单位/毫升（0.1毫升水）范围的浓度。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Yoshimitsu Kakuta et al.
Glycobiology, 18(1), 66-73 (2007-10-27)
Sialyltransferases are a family of glycosyltransferases that catalyze the transfer of N-acetylneuraminic acid residues from cytidine monophosphate N-acetylneuraminic acid (CMP-NeuAc) as a donor substrate to the carbohydrate groups of glycoproteins and glycolipids as acceptor substrates. We determined the crystal structure
Vireak Thon et al.
Applied microbiology and biotechnology, 94(4), 977-985 (2011-11-15)
Pasteurella multocida (Pm) strain Pm70 has three putative sialyltransferase genes including Pm0188, Pm0508, and Pm1174. A Pm0188 gene homolog in Pm strain P-1059 encodes a multifunctional α2-3-sialyltransferase, PmST1, that prefers oligosaccharide acceptors. A Pm0508 gene homolog in the same strain
Sung-Wook Son et al.
Biochemical and biophysical research communications, 414(1), 159-164 (2011-09-29)
In this study we investigated for the first time the transcriptional regulation of pig Galβ1,3GalNAc α2,3-sialyltransferase (pST3Gal I) in response to TGF-β1 in porcine kidney PK-15 cells. The pST3Gal I gene was found to span about 90kb and to be
Peng Zhang et al.
Bipolar disorders, 12(8), 786-792 (2010-12-24)
Multiple linkage and association studies have suggested chromosome 8q24 as a promising candidate region for bipolar disorder (BP). We performed a detailed association analysis assessing the contribution of common genetic variation in this region to the risk of BP. We
Vishwanath B Chachadi et al.
The international journal of biochemistry & cell biology, 43(4), 586-593 (2010-12-21)
Sialyl Lewis X is a tumor-associated antigen frequently found in the advanced cancers. However, the mechanism for the production of this cancer antigen is not entirely clear. The objective of this study is to examine whether epigenetics is involved in
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Enzymatic glycosyltransferase specificity challenges the one enzyme-one linkage concept.
Explore tools for glycosyltransferase synthesis and modification of glycans, such as glycosyltransferases and nucleotide sugar donors.
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
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