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重组
expressed in E. coli BL21
质量水平
表单
lyophilized powder
比活
≥3.0 units/mg protein
分子量
33.4 kDa
运输
dry ice
储存温度
−20°C
一般描述
唾液酸醛缩酶，或N-乙酰神经氨酸裂解酶，可催化N-乙酰神经氨酸的可逆醛醇裂解以形成丙酮酸和N-乙酰基-D-甘露糖胺。N-乙酰神经氨酸裂解酶在自然状态下主要存在于病原体中。
应用
唾液酸醛缩酶可用于合成C(6)至C(10)的非天然糖，以设计糖酶的拮抗剂和抑制剂。
单位定义
在37°C、pH 8.0条件下，一单位将会在每分钟内催化从Man-N-Ac和丙酮酸形成1.0 μmol Neu-5-Ac。
外形
含有Tris-HCl和NaCl的冻干粉
分析说明
酶活性测定在含有Neu-5-Ac (10 mM) 的Tris-HCl缓冲液（100 mM，pH 7.5）中于37 °C进行15分钟，并使用带有紫外检测器的毛细管电泳在200 nm进行分析。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Modulation of substrate specificities of D-sialic acid aldolase through single mutations of Val-251.
Chien-Yu Chou et al.
The Journal of biological chemistry, 286(16), 14057-14064 (2011-01-29)
In a recent directed-evolution study, Escherichia coli D-sialic acid aldolase was converted by introducing eight point mutations into a new enzyme with relaxed specificity, denoted RS-aldolase (also known formerly as L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase), which showed a preferred selectivity toward
Guiomar Sánchez-Carrón et al.
Applied and environmental microbiology, 77(7), 2471-2478 (2011-02-15)
N-Acetylneuraminate lyases (NALs) or sialic acid aldolases catalyze the reversible aldol cleavage of N-acetylneuraminic acid (Neu5Ac) to form pyruvate and N-acetyl-d-mannosamine (ManNAc). In nature, N-acetylneuraminate lyase occurs mainly in pathogens. However, this paper describes how an N-acetylneuraminate lyase was cloned
Jozef Nahálka et al.
Organic & biomolecular chemistry, 7(9), 1778-1780 (2009-07-11)
Active inclusion bodies of polyphosphate kinase 3 and cytidine 5'-monophosphate kinase were combined with whole cells that co-express sialic acid aldolase and CMP-sialic acid synthetase. The biocatalytic mixture was used for the synthesis of CMP-sialic acid, which was then converted
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