产品名称
链霉亲和素-琼脂糖 来源于阿维丁链霉菌, buffered aqueous suspension
form
buffered aqueous suspension
extent of labeling
≥1 mg per mL
technique(s)
affinity chromatography: suitable
matrix
4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
7 atoms
capacity
≥15 μg/mL binding capacity (biotin)
storage temp.
2-8°C
Quality Level
Application
来自链霉菌的链霉亲和−琼脂糖已用于：
- 在质膜转化生长因子β（TGFβ）受体II (TβRII)定量和Tβ
- RII内化过程中pull-down生物素化的细胞表面蛋白
- 生物素化miRNA pull-down实验；作为免疫沉淀的二级抗体
用于纯化含有蛋白质或DNA结合蛋白质的生物素
链亲和素琼脂糖用于蛋白质层析，亲和层析和重组蛋白表达和分析。链亲和素琼脂糖可以被用来研究烟草蚀纹病毒蛋白酶切割融合蛋白的定向固定。链亲和素琼脂糖被用来研发从天然植物提取物中筛选三重DNA结合物。
Biochem/physiol Actions
链霉亲和素是一种同型四聚体蛋白，分离自亲和素链霉菌中，与亲和素一样，对生物素具有很高的亲和力。链霉亲和素为轻度阴离子（pI ~ 5-6），非糖基化。与蛋清亲和素相比，这些特性使其非特异性结合力相对较低。链霉亲和素也比亲和素更不易被氯化胍分解成亚基。链霉亲和素-琼脂糖可用于固定或分离各种生物素化大分子和络合物（蛋白质、抗体、凝集素、核酸、受体和配体）。链霉亲和素-生物素相互作用需要苛刻的条件才能释放生物素化大分子。这一特性使得链霉亲和素-琼脂糖广泛用于各种亲和纯化应用。
Physical form
在pH7.2、含有0.05M NACl和0.02%叠氮化钠的0.01 M磷酸钠缓冲液中复溶
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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