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Merck
CN

S1452

Sigma-Aldrich

MISSION® siRNA Transfection Reagent

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别名:
MISSION® siRNA Transfection Reagent
UNSPSC代码:
41106502
NACRES:
NA.51

产品线

MISSION®

质量水平

形式

aqueous solution

运输

wet ice

储存温度

2-8°C

一般描述

MISSION® siRNA转染试剂适于转染siRNA到真核细胞,可实现≥90%的基因沉默达到瞬时基因敲除的效果。
使用低浓度的siRNA (1 nM)即可有效地沉默基因并降低脱靶效应。siRNA转染试剂以无菌溶液的形式提供,兼容血清和抗生素。

其他说明

基因沉默实验:采用24孔板、8.4 ng的21-mer荧光素酶siRNA、2 μL MISSION® siRNA转染试剂转染稳定表达萤火虫荧光素酶的A549细胞系。在存在血清的条件下分成三等分开展实验。转染后48小时,对细胞裂解液之中的荧光素酶表达情况进行评估。

法律信息

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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象形图

Flame

警示用语:

Warning

危险声明

危险分类

Flam. Liq. 3

WGK

WGK 3

闪点(°F)

120.2 °F

闪点(°C)

49 °C

法规信息

监管及禁止进口产品

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Tuyen T Dang et al.
Cancers, 12(7) (2020-07-11)
It was recently shown that the 5' to 3' exoribonuclease XRN2 is involved in the DNA damage response. Importantly, loss of XRN2 abrogates DNA double stranded break repair via the non-homologous end-joining pathway. However, the mechanistic details of how XRN2
Tuyen T Dang et al.
International journal of molecular sciences, 21(12) (2020-06-19)
Cellular survival is dependent on the efficient replication and transmission of genomic information. DNA damage can be introduced into the genome by several different methods, one being the act of DNA replication. Replication is a potent source of DNA damage
Ponnuraj Nagendraprabhu et al.
PLoS pathogens, 13(12), e1006779-e1006779 (2017-12-16)
Poxviruses have evolved multiple strategies to subvert signaling by Nuclear Factor κB (NF-κB), a crucial regulator of host innate immune responses. Here, we describe an orf virus (ORFV) virion-associated protein, ORFV119, which inhibits NF-κB signaling very early in infection (≤
Eirini Kefaloyianni et al.
Journal of the American Society of Nephrology : JASN, 30(12), 2370-2383 (2019-11-05)
Sustained activation of EGF receptor (EGFR) in proximal tubule cells is a hallmark of progressive kidney fibrosis after AKI and in CKD. However, the molecular mechanisms and particular EGFR ligands involved are unknown. We studied EGFR activation in proximal tubule
A E Witt et al.
Oncogene, 36(12), 1707-1720 (2016-10-04)
Tumours are comprised of a highly heterogeneous population of cells, of which only a small subset of stem-like cells possess the ability to regenerate tumours in vivo. These cancer stem cells (CSCs) represent a significant clinical challenge as they are

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