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一般描述
MISSION® siRNA转染试剂适于转染siRNA到真核细胞,可实现≥90%的基因沉默达到瞬时基因敲除的效果。
使用低浓度的siRNA (1 nM)即可有效地沉默基因并降低脱靶效应。siRNA转染试剂以无菌溶液的形式提供,兼容血清和抗生素。
使用低浓度的siRNA (1 nM)即可有效地沉默基因并降低脱靶效应。siRNA转染试剂以无菌溶液的形式提供,兼容血清和抗生素。
其他说明
基因沉默实验:采用24孔板、8.4 ng的21-mer荧光素酶siRNA、2 μL MISSION® siRNA转染试剂转染稳定表达萤火虫荧光素酶的A549细胞系。在存在血清的条件下分成三等分开展实验。转染后48小时,对细胞裂解液之中的荧光素酶表达情况进行评估。
法律信息
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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价格
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Warning
危险声明
危险分类
Flam. Liq. 3
储存分类代码
3 - Flammable liquids
WGK
WGK 3
闪点(°F)
120.2 °F
闪点(°C)
49 °C
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