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质量水平
描述
stability: stable to 0.2M NaOH
表单
suspension (20% aqueous ethanol)
特点
autoclavable
基质
crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
粒径
25-75 μm (wet)
25-75 μm (wet)
孔径
1000-100,000 Da MW range (globular proteins)
相容性
mode of use size exclusion chromatography
储存温度
2-8°C
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一般描述
- 肿胀，悬浮在 20% 乙醇水溶液中
- 高压灭菌
- 在 0.2 N 氢氧化钠 (NaOH) 中稳定
- 冷藏温度 (2-8°C) 下储存
应用
Sephacryl®已被用于：
- 开发用于镰状细胞疾病药物发现的新型高通量筛选测定（high-throughput screening assay）
- 绵羊胰腺弹性蛋白酶以及人尿中溶菌酶的纯化和表征
用于分子排阻色谱；HR级是较小的粒径，具有较窄的大小范围分布，能够以更快的流速实现更有效的分离。 已列出了球状蛋白质和葡聚糖的推荐分馏范围。
法律信息
Sephacryl is a registered trademark of Cytiva
警示用语：
Warning
危险声明
危险分类
Flam. Liq. 3
储存分类代码
3 - Flammable liquids
WGK
WGK 1
闪点(°F)
100.4 - 109.4 °F - closed cup
闪点(°C)
38 - 43 °C - closed cup
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
危险化学品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Matthew Lord et al.
The Journal of cell biology, 167(2), 315-325 (2004-10-27)
We purified native Myo2p/Cdc4p/Rlc1p (Myo2), the myosin-II motor required for cytokinesis by Schizosaccharomyces pombe. The Myo2p heavy chain associates with two light chains, Cdc4p and Rlc1p. Although crude Myo2 supported gliding motility of actin filaments in vitro, purified Myo2 lacked
David Novak et al.
Bioelectrochemistry (Amsterdam, Netherlands), 119, 136-141 (2017-10-11)
Cytochrome c (cyt c) is one of the most studied conjugated proteins due to its electron-transfer properties and ability to regulate the processes involved in homeostasis or apoptosis. Here we report an electrochemical strategy for investigating the electroactivity of cyt
Diederik Johannes Opperman et al.
Journal of bacteriology, 190(8), 3076-3082 (2008-02-12)
Bacteria can reduce toxic and carcinogenic Cr(VI) to insoluble and less toxic Cr(III). Thermus scotoductus SA-01, a South African gold mine isolate, has been shown to be able to reduce a variety of metals, including Cr(VI). Here we report the
Marc A Benson et al.
Clinica chimica acta; international journal of clinical chemistry, 411(3-4), 190-197 (2009-11-11)
Pseudomonas aeruginosa is an opportunistic pathogen that causes disease in immunocompromised individuals, burn victims, and cystic fibrosis patients. Strains that secrete ExoU induce host cell lysis and damage epithelial tissue, which can lead to severe outcomes including sepsis and mortality.
L Hagel et al.
Journal of chromatography, 476, 329-344 (1989-08-04)
The influence of experimental parameters on the separation result in gel filtration may readily be predicted from a few basic equations, as demonstrated with the aid of experimental observations on Sephacryl HR. Excellent agreement between the predicted resolution and that
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