产品名称
Sephacryl®, 100-HR, MW range 1000-100,000 Da (globular proteins)
description
stability: stable to 0.2M NaOH
form
suspension (20% aqueous ethanol)
feature
autoclavable
matrix
crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
particle size
25-75 μm (wet)
25-75 μm (wet)
pore size
1000-100,000 Da MW range (globular proteins)
compatibility
mode of use size exclusion chromatography
storage temp.
2-8°C
Quality Level
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Application
Sephacryl®已被用于:
- 开发用于镰状细胞疾病药物发现的新型高通量筛选测定(high-throughput screening assay)
- 绵羊胰腺弹性蛋白酶以及人尿中溶菌酶的纯化和表征
用于分子排阻色谱;HR级是较小的粒径,具有较窄的大小范围分布,能够以更快的流速实现更有效的分离。 已列出了球状蛋白质和葡聚糖的推荐分馏范围。
General description
- 肿胀,悬浮在 20% 乙醇水溶液中
- 高压灭菌
- 在 0.2 N 氢氧化钠 (NaOH) 中稳定
- 冷藏温度 (2-8°C) 下储存
Legal Information
Sephacryl is a registered trademark of Cytiva
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
存储类别
3 - Flammable liquids
wgk
WGK 1
flash_point_f
100.4 - 109.4 °F - closed cup
flash_point_c
38 - 43 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
危险化学品
此项目有
Matthew Lord et al.
The Journal of cell biology, 167(2), 315-325 (2004-10-27)
We purified native Myo2p/Cdc4p/Rlc1p (Myo2), the myosin-II motor required for cytokinesis by Schizosaccharomyces pombe. The Myo2p heavy chain associates with two light chains, Cdc4p and Rlc1p. Although crude Myo2 supported gliding motility of actin filaments in vitro, purified Myo2 lacked
Diederik Johannes Opperman et al.
Journal of bacteriology, 190(8), 3076-3082 (2008-02-12)
Bacteria can reduce toxic and carcinogenic Cr(VI) to insoluble and less toxic Cr(III). Thermus scotoductus SA-01, a South African gold mine isolate, has been shown to be able to reduce a variety of metals, including Cr(VI). Here we report the
David Novak et al.
Bioelectrochemistry (Amsterdam, Netherlands), 119, 136-141 (2017-10-11)
Cytochrome c (cyt c) is one of the most studied conjugated proteins due to its electron-transfer properties and ability to regulate the processes involved in homeostasis or apoptosis. Here we report an electrochemical strategy for investigating the electroactivity of cyt
Marc A Benson et al.
Clinica chimica acta; international journal of clinical chemistry, 411(3-4), 190-197 (2009-11-11)
Pseudomonas aeruginosa is an opportunistic pathogen that causes disease in immunocompromised individuals, burn victims, and cystic fibrosis patients. Strains that secrete ExoU induce host cell lysis and damage epithelial tissue, which can lead to severe outcomes including sepsis and mortality.
M L Galisteo et al.
Biophysical journal, 65(1), 227-235 (1993-07-01)
During the packaging of double-stranded DNA by bacterial viruses, the precursor procapsid loses its internal core of scaffolding protein and undergoes a substantial expansion to form the mature virion. Here we show that upon heating, purified P22 procapsids release their
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