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Merck
CN

RNB300

Sigma-Aldrich

GenElute 单细胞RNA纯化试剂盒

greener alternative

别名:

RNA extraction kit for NGS, RNA extraction kit for qPCR, RNA isolation kit for NGS, RNA isolation kit for qPCR, RNA purification kit for NGS, RNA purification kit for qPCR, single cell RNA extraction kit, single cell RNA isolation kit, single cell RNA kit, single cell total RNA extraction kit, single cell total RNA isolation kit, single cell total RNA purification kit

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About This Item

UNSPSC代码:
41105600
NACRES:
NA.52

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

环保替代产品分类

运输

ambient

储存温度

room temp

一般描述

Sigma GenElute 单细胞RNA纯化试剂盒用于从少量的培养动物细胞、分选细胞和微切割样本(包括激光捕获显微切割(LCM)样本)中快速灵敏地分离和纯化总RNA。该试剂盒可从单个细胞至2 x 10^5个细胞中回收RNA。该试剂盒可纯化任何大小的RNA,从较大的mRNA和rRNA到miRNA和小干扰RNA (siRNA)均可分离。该试剂盒无需使用苯酚或氯仿即可从蛋白质等其他细胞组分中优先纯化出RNA。纯化得到的RNA完整性高,适用于多种下游应用,比如实时定量PCR、逆转录PCR、Northern杂交、RNase保护、引物延伸及表达阵列分析。

  • 快速微型离心柱型式,处理过程方便快捷
  • 洗脱体积小,仅有8 μL
  • 在不影响总得率的前提下,分离出总RNA(从较大的rRNA到miRNA均可分离)
  • 无需苯酚或氯仿提取
  • 可从多种来源样本分离得到优质总RNA
  • 可从低至单个动物细胞的样本中分离并检测出RNA

应用

  • 定量,大mRNA和小RNA(包括miRNA)的实时定量RT-PCR
  • 大mRNA和小RNA(包括miRNA)的RT-PCR
  • 表达谱
  • RNA 和miRNA 的二代测序
  • microRNA 克隆和扩增
  • Northern杂交
  • RNase保护
  • 引物延伸
  • miRNA阵列
  • RNA-Seq
  • Nanostring
  • Fluidigm
  • Droplet/Digital PCR

特点和优势

  • 非常适合低起始量样本 - 可从少量的培养动物细胞、分选细胞和微切割样本(包括激光捕获显微切割 (LCM) 样本)中分离总RNA。可从低至单个细胞的样本中分离RNA。
  • 洗脱体积小 – 可采用低至8 μL的洗脱液洗脱总RNA
  • 无需苯酚:氯仿提取 - 无需使用苯酚或氯仿等有害化学成分即可分离总RNA
  • 可分离多种类型的RNA – 在不影响总得率的前提下,分离出全部类型的RNA,从较大的mRNA和rRNA到miRNA和小干扰RNA (siRNA)均可分离
  • 处理过程方便快捷 - 使用快速的离心柱,可在20分钟内处理10个样本

单位定义

制备

其他说明

我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用苯酚和氯仿的标准DNA提取方法,该产品是一种更安全的化学产品。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

象形图

CorrosionExclamation mark

警示用语:

Danger

危险分类

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

补充剂危害

储存分类代码

8A - Combustible corrosive hazardous materials

WGK

WGK 2


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访问文档库

Shasha Zhang et al.
Cellular and molecular life sciences : CMLS, 77(7), 1401-1419 (2019-09-06)
Foxg1 is one of the forkhead box genes that are involved in morphogenesis, cell fate determination, and proliferation, and Foxg1 was previously reported to be required for morphogenesis of the mammalian inner ear. However, Foxg1 knock-out mice die at birth
Tao Gao et al.
JCI insight, 6(10) (2021-04-14)
Retinoic acid (RA) signaling is essential for enteric nervous system (ENS) development, since vitamin A deficiency or mutations in RA signaling profoundly reduce bowel colonization by ENS precursors. These RA effects could occur because of RA activity within the ENS
Yuxian Guo et al.
Nature communications, 12(1), 7094-7094 (2021-12-09)
Oxidative stress contributes to the pathogenesis of acute lung injury. Protein S-glutathionylation plays an important role in cellular antioxidant defense. Here we report that the expression of deglutathionylation enzyme Grx1 is decreased in the lungs of acute lung injury mice.
Dandan Liang et al.
Nature communications, 12(1), 287-287 (2021-01-14)
Bioelectrical impulses intrinsically generated within the sinoatrial node (SAN) trigger the contraction of the heart in mammals. Though discovered over a century ago, the molecular and cellular features of the SAN that underpin its critical function in the heart are
Shaoxue Zeng et al.
Theranostics, 12(15), 6705-6722 (2022-10-04)
Rationale: Müller cells play an essential role in maintaining the health of retinal photoreceptors. Dysfunction of stressed Müller cells often results in photoreceptor degeneration. However, how these cells communicate under stress and the signalling pathways involved remain unclear. In this

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