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重组
expressed in E. coli
质量水平
检测方案
≥80% (SDS-PAGE)
形式
buffered aqueous glycerol solution
分子量
28 kDa
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... RASGEF1B(153020)
一般描述
Ras proto-oncogene is a guanine-nucleotide binding protein that couples tyrosine kinase receptor signals to mitogen activated protein (MAP) kinase cascade. It has an important role in cell growth. The Ras gene is localized on human chromosome 11p15.5. Ras is a substrate for farnesyltransferase (FTase) and when farnesylated, it is anchored to the membrane.
应用
Human Ras has been used as a negative control in tubulin overlay assay.
生化/生理作用
Ras proteins, p21, are guanyl nucleotide binding proteins that play an important role in cellular signal transduction. Ras proteins are regulatory proteins that bind GTP in their active state. A Ras intrinsic GTPase activity hydrolyzes guanosine-5′-triphosphate (GTP) to guanosine diphosphate (GDP) thus inactivating Ras, while activation of Ras requires exchange of the bound GDP with GTP. Inhibition of Ras activity by stimulation of its GTPase activity is enhanced by GTPase activating proteins. Membrane localization of Ras is crucial for its cellular activity. Inhibition of Ras farnesylation is a target for anti-cancer drug development, since abnormally active Ras is present in more than 50% of human cancers.
包装
Package size based on protein content
外形
Solution in 50% glycerol containing 50 mM Tris (pH 8.0), 5 mM MgCl2, 45 mM NaCl, 1 mM DTT
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Real-time in vitro measurement of intrinsic and Ras GAP-mediated GTP hydrolysi
Test (2006)
Inactivation of Ras GTPase-activating proteins promotes unrestrained activity of wild-type Ras in human liver cancer
Journal of Hepatology, 54(2), 311-311 (2011)
Real-time in vitro measurement of intrinsic and Ras GAP-mediated GTP hydrolysi
Methods in Enzymology (2006)
The EMBO journal, 5(6), 1351-1358 (1986-06-01)
v-Ha-ras encoded p21 protein (p21V), the cellular c-Ha-ras encoded protein (p21C) and its T24 mutant form p21T were produced in Escherichia coli under the control of the tac promoter. Large amounts of the authentic proteins in a soluble form can
Human Mucin Gene MUC5B, the 10.7-kb Large Central Exon Encodes Various Alternate Subdomains Resulting in a Super-repeat
The Journal of Biological Chemistry (1997)
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