grade
Molecular Biology
type
Type V
form
lyophilized powder
specific activity
15-19 units/mg solid
storage condition
desiccated
solubility
H2O: ~2 mg/mL, clear to hazy
storage temp.
2-8°C
Quality Level
General description
麦胚 tRNA 含有许多不同的特异性 tRNA 分子。
Application
粗转移 RNA (tRNA) 从小麦胚芽中提纯，含有许多特定的 tRNA 分子，可以通过不同的过程分离。利用 体外 翻译系统进行详细的结构和功能研究实验通常需要高纯度的 tRNA，这可以通过色谱分离和 tRNA 的可逆生化修饰的组合来实现。麦胚 tRNA 已用于从麦胚纯化 RNAse Z（tRNA 3′-处理核酸内切酶）。纯化包括 6 个步骤，其中最有效的步骤是使用由麦胚 tRNA 构成的亲和柱，其与 RNAse Z 紧密结合。
适合作为核酸纯化和沉淀中的载体使用。
Preparation Note
tRNA 以冻干粉形式提供，可溶于水，浓度为 2 mg/mL。
Other Notes
在 1.0 mL 水中（1 cm 光径），1 个单位在 260 nm 处的吸光度为 1.0。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Spatial and temporal expression of the zebrafish genome by large-scale in situ hybridization screening.
Bernard Thisse et al.
Methods in cell biology, 77, 505-519 (2004-12-18)
Christèle Maison et al.
Nature genetics, 30(3), 329-334 (2002-02-19)
Post-translational modification of histone tails is thought to modulate higher-order chromatin structure. Combinations of modifications including acetylation, phosphorylation and methylation have been proposed to provide marks recognized by specific proteins. This is exemplified, in both mammalian cells and fission yeast
Yana P Blokhina et al.
PLoS genetics, 15(1), e1007730-e1007730 (2019-01-18)
Meiosis is a cellular program that generates haploid gametes for sexual reproduction. While chromosome events that contribute to reducing ploidy (homologous chromosome pairing, synapsis, and recombination) are well conserved, their execution varies across species and even between sexes of the
Fu-Jung Lin et al.
PLoS genetics, 9(5), e1003516-e1003516 (2013-05-30)
Mouse Ikbkap gene encodes IKAP--one of the core subunits of Elongator--and is thought to be involved in transcription. However, the biological function of IKAP, particularly within the context of an animal model, remains poorly characterized. We used a loss-of-function approach
Sichen Shao et al.
Molecular cell, 50(5), 637-648 (2013-05-21)
Quality control of defective mRNAs relies on their translation to detect the lesion. Aberrant proteins are therefore an obligate byproduct of mRNA surveillance and must be degraded to avoid disrupting protein homeostasis. These defective translation products are thought to be
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