推荐产品
产品名称
RPMI-1640 培养基, Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes
运输
ambient
储存温度
2-8°C
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一般描述
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
RPMI-1640培养基适用于人正常白细胞和肿瘤白细胞=培养。
应用
RPMI-1640培养基已用于：
- 培养套细胞淋巴瘤细胞
- 培养小鼠足细胞
- 提取大鼠股骨和胫骨全骨髓
其他说明
已证明酚红在克隆密度时干扰一些细胞的生长。
在处理干细胞或低密度培养细胞时使用该培养基。
也推荐用于体外诊断。
在处理干细胞或低密度培养细胞时使用该培养基。
也推荐用于体外诊断。
补充剂
通常也和此产品一起购买
产品编号
说明
价格
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Ingvild S M Gabrielsen et al.
PloS one, 14(7), e0218858-e0218858 (2019-07-02)
Antigen presenting cells (APCs) in the thymus play an essential role in the establishment of central tolerance, i.e. the generation of a repertoire of functional and self-tolerant T cells to prevent autoimmunity. In this study, we have compared the transcriptomes
Ida Marie Rundgren et al.
Journal of immunological methods, 461, 53-62 (2018-06-16)
Monocytes are important for innate immunity and include the classical (CD14brightCD16negative), intermediate (CD14brightCD16dim) and non-classical (CD14dimCD16bright) monocyte subsets. The quantification of these functionally different subsets in peripheral blood may become useful for diagnosis and follow-up in human diseases. The aim
Deepak Kumar et al.
Aging, 14(5), 2081-2100 (2022-03-02)
Alterations in RNA splicing are associated with different malignancies, including leukemia, lymphoma, and solid tumors. The RNA splicing modulators such as FD-895 and pladienolide B have been investigated in different malignancies to target/modulate spliceosome for therapeutic purpose. Different cell lines
Alyson A Fiorillo et al.
Frontiers in immunology, 11, 151-151 (2020-03-11)
MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate important intracellular biological processes. In myasthenia gravis (MG), a disease-specific pattern of elevated circulating miRNAs has been found, and proposed as potential biomarkers. These elevated miRNAs include miR-150-5p, miR-21-5p, and miR-30e-5p
Zhihong Lu et al.
Cytotechnology, 73(5), 697-713 (2021-10-12)
Protein phosphatase 2A (PP2A) is one of the major protein serine/threonine phosphatases (PPPs) with regulatory effects on several cellular processes, but its role and function in Adriamycin (ADR)-treated podocytes injury needs to be further explored. Mice podocytes were treated with
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