product name
RPMI-1640 培养基, Modified, with 20 mM HEPES and L-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
形式
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
pH值（酸碱度）
>7.2
组分
HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes
运输
ambient
储存温度
2-8°C
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一般描述
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
RPMI-1640培养基配方基于RPMI-1630系列培养基，利用碳酸氢盐缓冲系统和氨基酸和维生素数量的改变。用于人正常白细胞和肿瘤白细胞的培养。RPMI-1640还支持各种类型的细胞培养物的生长，如72小时植物血凝素(PHA)刺激试验中的新鲜人淋巴细胞。
应用
RPMI-1640培养基已用于培养：
- 人永生角化细胞、体外转染试验
- 细胞系和人T细胞母细胞，监控自噬流量
- 小猪外周血单核细胞(PBMC)
补充剂
通常也和此产品一起购买
产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
Patrick Linder et al.
PLoS genetics, 10(2), e1004207-e1004207 (2014-03-04)
RNA decay and maturation have in recent years been recognised as major regulatory mechanisms in bacteria. In contrast to Escherichia coli, the Firmicute (Gram-positive) bacteria often do not encode the well-studied endonuclease RNase E, but instead rely on the endonucleases
Višnja Stepanić et al.
Antioxidants (Basel, Switzerland), 8(4) (2019-04-10)
Chalcones are polyphenolic secondary metabolites of plants, many of which have antioxidant activity. Herein, a set of 26 synthetic chalcone derivatives with alkyl substituted pyrazine heterocycle A and four types of the monophenolic ring B, were evaluated for the potential
Janina Treffon et al.
Cellular microbiology, 22(5), e13158-e13158 (2020-01-03)
Staphylococcus aureus is one of the earliest pathogens that persists the airways of cystic fibrosis (CF) patients and contributes to increased inflammation and decreased lung function. In contrast to other staphylococci, S. aureus possesses two superoxide dismutases (SODs), SodA and
Christine Silvia Gibhardt et al.
Cell reports, 33(3), 108292-108292 (2020-10-22)
Store-operated calcium entry (SOCE) through STIM-gated ORAI channels governs vital cellular functions. In this context, SOCE controls cellular redox signaling and is itself regulated by redox modifications. However, the molecular mechanisms underlying this calcium-redox interplay and the functional outcomes are
Maarit Suomalainen et al.
Journal of cell science, 134(5) (2020-09-13)
In clonal cultures, not all cells are equally susceptible to virus infection, and the mechanisms underlying this are poorly understood. Here, we developed image-based single-cell measurements to scrutinize the heterogeneity of adenovirus (AdV) infection. AdV delivers, transcribes and replicates a
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