R7388
RPMI-1640 培养基
With HEPES, with ʟ-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
别名:
Roswell Park Memorial Institute 1640 medium
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产品名称
RPMI-1640 培养基, Modified, with 20 mM HEPES and L-glutamine, without sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
>7.2
components
HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
RPMI-1640培养基配方基于RPMI-1630系列培养基，利用碳酸氢盐缓冲系统和氨基酸和维生素数量的改变。用于人正常白细胞和肿瘤白细胞的培养。RPMI-1640还支持各种类型的细胞培养物的生长，如72小时植物血凝素(PHA)刺激试验中的新鲜人淋巴细胞。
Application
RPMI-1640培养基已用于培养：
- 人永生角化细胞、体外转染试验
- 细胞系和人T细胞母细胞，监控自噬流量
- 小猪外周血单核细胞(PBMC)
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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RNA decay and maturation have in recent years been recognised as major regulatory mechanisms in bacteria. In contrast to Escherichia coli, the Firmicute (Gram-positive) bacteria often do not encode the well-studied endonuclease RNase E, but instead rely on the endonucleases
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Staphylococcus aureus is one of the earliest pathogens that persists the airways of cystic fibrosis (CF) patients and contributes to increased inflammation and decreased lung function. In contrast to other staphylococci, S. aureus possesses two superoxide dismutases (SODs), SodA and
Christine Silvia Gibhardt et al.
Cell reports, 33(3), 108292-108292 (2020-10-22)
Store-operated calcium entry (SOCE) through STIM-gated ORAI channels governs vital cellular functions. In this context, SOCE controls cellular redox signaling and is itself regulated by redox modifications. However, the molecular mechanisms underlying this calcium-redox interplay and the functional outcomes are
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| R7388-6X500ML | 04061836696306 |
| R7388-500ML | 04061836696276 |
| R7388-1L | 04061836696269 |
| R7388-100ML | 04061836696238 |