特异性
识别序列： 5′-G/AATTC-3′
切割结果：1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活： 65°C，20 min。
切割结果：1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活： 65°C，20 min。
应用
Eco RI 是一种限制性内切酶，在分子生物学应用中用于切割识别站点 5′ 的 DNA-G/AATTC-3′，产生带有 5′ 的片段-粘性终点。
其他说明
提供 10x 限制性内切酶缓冲液 SH (B3657)。
备注： 避免次优反应条件，如低盐、高 pH 值 ( 8.0) 和高甘油 ( 5%)，因为它们将改变 EcoRI 特异性并沉淀星号活性。
单位定义
一个单位表示在37°C下1小时内，在总体积为50 ml的1x限制酶消化缓冲液SH中完全切割1 mg λDNA的酶活性。1 mg pBR322 DNA可通过2个单位的EcoR I完全消化。
外形
10 mM Tris-HCl 溶液，pH 值 7.2、1 mM EDTA、200 mM NaCl、0.5 mM 二硫赤藓糖醇、50% 甘油 (v/v)、0.2%Triton X-100 (v/v)，4°C
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
J Hedgpeth et al.
Proceedings of the National Academy of Sciences of the United States of America, 69(11), 3448-3452 (1972-11-01)
The sequence of DNA base pairs adjacent to the phosphodiester bonds cleaved by the RI restriction endonuclease in unmodified DNA from coliphage lambda has been determined. The 5'-terminal nucleotide labeled with (32)P and oligonucleotides up to the heptamer were analyzed
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Yu-Feng Huang et al.
BMC systems biology, 6 Suppl 2, S10-S10 (2013-01-11)
Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
Rachel M Smith et al.
Nucleic acids research, 41(1), 391-404 (2012-11-14)
Type IIB restriction-modification systems, such as BcgI, feature a single protein with both endonuclease and methyltransferase activities. Type IIB nucleases require two recognition sites and cut both strands on both sides of their unmodified sites. BcgI cuts all eight target
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