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特异性
识别序列: 5′-G/AATTC-3′
切割结果:1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活: 65°C,20 min。
切割结果:1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活: 65°C,20 min。
应用
Eco RI 是一种限制性内切酶,在分子生物学应用中用于切割识别站点 5′ 的 DNA-G/AATTC-3′,产生带有 5′ 的片段-粘性终点。
其他说明
提供 10x 限制性内切酶缓冲液 SH (B3657)。
备注: 避免次优反应条件,如低盐、高 pH 值 ( 8.0) 和高甘油 ( 5%),因为它们将改变 EcoRI 特异性并沉淀星号活性。
单位定义
一个单位表示在37°C下1小时内,在总体积为50 ml的1x限制酶消化缓冲液SH中完全切割1 mg λDNA的酶活性。1 mg pBR322 DNA可通过2个单位的EcoR I完全消化。
外形
10 mM Tris-HCl 溶液,pH 值 7.2、1 mM EDTA、200 mM NaCl、0.5 mM 二硫赤藓糖醇、50% 甘油 (v/v)、0.2%Triton X-100 (v/v),4°C
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
Proceedings of the National Academy of Sciences of the United States of America, 69(11), 3448-3452 (1972-11-01)
The sequence of DNA base pairs adjacent to the phosphodiester bonds cleaved by the RI restriction endonuclease in unmodified DNA from coliphage lambda has been determined. The 5'-terminal nucleotide labeled with (32)P and oligonucleotides up to the heptamer were analyzed
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
TALENs and ZFNs are associated with different mutation signatures.
Nature methods, 10(3), 185-185 (2013-02-12)
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