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Merck
CN

R5886

SAFC

RPMI-1640 培养基

HEPES modification, without ʟ-glutamine, sterile liquid, suitable for cell culture

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别名:
Roswell Park Memorial Institute 1640 medium
MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

pH值(酸碱度)

>7.2

组分

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes

运输

ambient

储存温度

2-8°C

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一般描述

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
该RPMI-1640培养基补充了HEPES。 HEPES是一种Good′s生理缓冲液,有助于提供比碳酸氢盐缓冲液更稳定的培养基pH。

应用

RPMI-1640培养基已用于:
  • 培养人淋巴母细胞系细胞
  • 前列腺癌细胞
  • 人血单核细胞

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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