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生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~24 kDa
种属反应性
human, rat, mouse
浓度
~1.0 mg/mL
技术
indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 and rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.1% and 0.4% saponin, respectively.
western blot: 2-5 μg/mL using whole extract of human HEK-293T cells
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... RAB4A(5867)
mouse ... Rab4a(19341)
rat ... Rab4a(25532)
一般描述
Rab4 is a GTPase , that is located in early and recycling endosomes. It belongs to Rab family and is located on human chromosome 1q42. In rat testis, Rab4 is found in sertoli and germ cells.
There are two highly homologous RAB4 isoforms, RAB4A and RAB4B, encoded by two separate genes (RAB4A and RAB4B). RAB4A and RAB4B are localized in the same cellular compartments and are believed to have similar or identical functions in recycling.
应用
The antibody can be used in several immunochemical techniques including immunoblotting (24 kDa) and immunofluorescence.
生化/生理作用
Rab4 regulates endocytic recycling in a cell cycle dependent fashion. Rab4 is associated with early endosomes and recycling endosomes and regulates the recycling of membranes and proteins from these compartments back to the plasma membrane. Rab4 interacts with several Rab4 effector proteins like Rabip4, that regulates integrin trafficking and is involved in the migration of NIH 3T3 fibroblasts.
Rab4a controls integrin trafficking throughout cell adhesion. It helps in the quick reprocess of receptors from early endosomes to the cell surface. It also controls the secretion of procathepsin L in human melanoma cells.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
The Rab4A effector protein Rabip4 is involved in migration of NIH 3T3 fibroblasts
Vukmirica J, et al.
The Journal of Biological Chemistry, 281(47), 36360-36368 (2006)
Procathepsin L secretion, which triggers tumour progression, is regulated by Rab4a in human melanoma cells
Barbarin A and Frade R
The Biochemical Journal, 437(1), 97-107 (2011)
Expression of RAB4B, a protein governing endocytic recycling, is co-regulated with MHC class II genes
Krawczyk M, et al.
Nucleic Acids Research, 35(2), 595-605 (2006)
Rab4A GTPase catenin interactions are involved in cell junction dynamics in the testis
Mruk DD, et al.
Journal of Andrology, 28(5), 742-754 (2007)
TBC1D16 is a Rab4A GTPase activating protein that regulates receptor recycling and EGF receptor signaling
Goueli BS, et al.
Proceedings of the National Academy of Sciences of the USA, 109(39), 15787-15792 (2012)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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