生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~58 kDa
种属反应性
mouse, rat, human
浓度
~1.0 mg/mL
技术
indirect immunofluorescence: 5-10 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin
western blot: 2-5 μg/mL using whole extracts of mouse brain and human U87 cells
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... RABGEF1(27342)
mouse ... Rabgef1(56715)
rat ... Rabgef1(360797)
一般描述
Anti-Rabex-5 (N-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to a sequence at N-terminal of human Rabex-5, with an added cysteine, conjugated to KLH. Rabex-5 contains multiple functional domains, including a vacuolar protein sorting-associated (VPS9) domain that possesses the GDP/GTP exchange factors (GEF) activity, a ubiquitin-binding A20-like zinc finger (ZnF) domain, and a coiled-coil domain.
应用
Anti-Rabex-5 (N-terminal) antibody may be used in immunoblotting and immunofluorescence.
生化/生理作用
Rabex-5 forms a tight complex with Rabaptin-5 that is essential for endocytic membrane fusion. The bound Rabaptin-5 to Rabex-5 increases the GEF activity of Rabex-5 and fastens the generation of the active GTP-bound form of membrane associated Rab5.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain
Mattera R, et al.
Test, 281(10), 6874-6883 (2006)
Kavitha Balaji et al.
Traffic (Copenhagen, Denmark), 15(11), 1206-1218 (2014-08-02)
Listeria monocytogenes is a food-borne pathogenic bacterium that invades intestinal epithelial cells through a phagocytic pathway that relies on the activation of host cell RAB5 GTPases. Listeria monocytogenes must subsequently inhibit RAB5, however, in order to escape lysosome-mediated destruction. Relatively
Functional synergy between Rab5 effector Rabaptin-5 and exchange factor Rabex-5 when physically associated in a complex
Lippe R, et al.
Molecular Biology of the Cell, 12(7), 2219-2228 (2001)
The RAB5-GEF Function of RIN1 Regulates Multiple Steps During Listeria monocytogenes Infection.
Kavitha Balaji et al.
Traffic (Copenhagen, Denmark), 16(7), 796-796 (2015-06-09)
David Millrine et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(38), 10625-10630 (2016-09-08)
Immunomodulatory drugs (IMiDs) are a family of compounds derived from thalidomide. Binding of the IMiD molecule to the Lon protease Cereblon initiates the degradation of substrates via the ubiquitin proteasome pathway. Here, we show that Cereblon forms a complex with
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