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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
4D11, monoclonal
形式
buffered aqueous solution
分子量
antigen ~68 kDa
种属反应性
bovine, newt, chicken, human, monkey, mouse
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of MDBK cells
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... HNRNPL(3191)
mouse ... Hnrnpl(15388)
一般描述
Monoclonal Anti-hnRNP-L (mouse IgG1 isotype) is derived from the 4D11 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALA/c mice immunized with hnRNP proteins and purified by affinity chromatography on ssDNA-agarose. RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). hnRNPs consist of protein groups named A to U and many of these protein groups consist of more than one isoform. Group L and M are one of the most abundant groups of proteins.
免疫原
hnRNP proteins.
应用
Anti-hnRNP-L antibody has been used in
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
- immunocytochemistry
- western blotting
生化/生理作用
Heterogeneous nuclear ribonucleoproteins-L (hnRNP-L) is involved in splicing regulations, internal translation initiation of hepatitis C virus (HCV), stability of the human vascular endothelial growth factor (VEGF) mRNA under hypoxic growth conditions, and intron independent mRNA export.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression.
Liu X and Mertz J E
Genes & Development, 9(14), 1766-1780 (1995)
Auto-and cross-regulation of the hnRNP L proteins by alternative splicing
RossbachO, et al.
Molecular and cellular biology, 29(6), 1442-1451 (2009)
Regulation of human vascular endothelial growth factor mRNA stability in hypoxia by heterogeneous nuclear ribonucleoprotein L
Shih S C and Claffey K P
The Journal of Biological Chemistry, 274(3), 1359-1365 (1999)
Vineetha Mathew et al.
Gene expression patterns : GEP, 48, 119319-119319 (2023-05-07)
Heterogeneous nuclear ribonucleoprotein L (hnRNPL) is a conserved RNA binding protein (RBP) that plays an important role in the alternative splicing of gene transcripts, and thus in the generation of specific protein isoforms. Global deficiency in hnRNPL in mice results
Matthew S Alexander et al.
Muscle & nerve, 63(6), 928-940 (2021-03-03)
RNA-binding proteins (RBPs) play an important role in skeletal muscle development and disease by regulating RNA splicing. In myotonic dystrophy type 1 (DM1), the RBP MBNL1 (muscleblind-like) is sequestered by toxic CUG repeats, leading to missplicing of MBNL1 targets. Mounting
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