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生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~25 kDa
种属反应性
human, rat
浓度
~1.5 mg/mL
技术
indirect immunofluorescence: 5-10 μg/mL using rat NRK cells fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin
western blot: 2-5 μg/mL using whole extract of human A431 cells
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... RAB27A(5873)
rat ... Rab27a(50645)
一般描述
Rab27 (Ras-related protein) is a member of the Rab family of small guanosine triphosphatases (GTPases). Rab27 is widely expressed in non-neuronal regulated secretory cells, including exocrine, endocrine, ovarian, and hematopoietic cells.
Rab27A (Ras-related protein) belongs to the Rab family of small GTPase proteins. It is located on chromosome 15q21.
免疫原
synthetic peptide corresponding to amino acid residues 45-57 of human RAB27A, with an added cysteine, conjugated to KLH. The corresponding sequence differs by one amino acid in rat and mouse Rab27A and by 2 amino acids in human RAB27B.
应用
Anti-Rab27 (N-terminal) antibody has been used in immunoblotting, immunofluorescence.
生化/生理作用
In humans, Rab27A (Ras-related protein) plays important roles in the development of cancers. It is considered as a prognostic biomarker for CRC (colorectal cancer) patients. It may participate in the transfer of protein and signal transduction mediated by GTPase. Rab27 mutation is linked with Griscelli syndrome type 2. It is also responsible for the innate immune resistance against invading microorganisms.
Rab27 (Ras-related protein) regulates the exocytosis of these cell-specific store organelles, using multiple organelle-specific effector proteins, such as granuphilin and melanophilin.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
Rab27a was identified as a prognostic biomaker by mRNA profiling, correlated with malignant progression and subtype preference in gliomas
Wang H, et al.
PLoS ONE, 9(2) (2014)
High Rab27A expression indicates favorable prognosis in CRC
Shi C, et al.
Diagnostic Pathology, 10:68 (2015)
Bong Hwan Sung et al.
Nature communications, 6, 7164-7164 (2015-05-15)
Directional cell movement through tissues is critical for multiple biological processes and requires maintenance of polarity in the face of complex environmental cues. Here we use intravital imaging to demonstrate that secretion of exosomes from late endosomes is required for
Hironori Higashio et al.
Scientific reports, 6, 22539-22539 (2016-03-05)
Mast cell degranulation is regulated by the small guanosine triphosphatases (GTPases) Rab27a and Rab27b, which have distinct and opposing roles: Rab27b acts as a positive regulator through its effector protein Munc13-4, a non-neuronal isoform of the vesicle-priming Munc13 family of
Rab27 effectors, pleiotropic regulators in secretory pathways
Fukuda M, et al.
Traffic, 14(9), 949-963 (2013)
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