生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 48 kDa
种属反应性
human
技术
indirect immunofluorescence: 1-2 μg/mL using HeLa cells fixed with paraformaldehyde-Triton
western blot: 0.5-1 μg/mL using MCF7 nuclear extracts
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... RBBP4(5928)
mouse ... Rbbp4(19646)
rat ... Rbbp4(313048)
一般描述
Histone binding protein (RbAp48, also known as also known as RBBP4 or NURF55) contains 425 amino acids, tryptophan-aspartic acid (WD)-domain containing protein isolated as a retinoblastoma binding protein (RB1).
免疫原
synthetic peptide corresponding to amino acids 1-14 of human RbAp48, conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide is conserved in human, mouse, and rat.
应用
Anti-RbAp48 antibody produced in rabbit has been used in immunoprecipitation and western blot analysis. It may also be used in immunofluorescence.
生化/生理作用
RbAp48 is found in association with chromatin remodeling complexes Sin3A/histone deacetylase (HDAC) and NURD (nucleosomal remodeling and deacetylation complex).
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
SAP30L interacts with members of the Sin3A corepressor complex and targets Sin3A to the nucleolus
Viiri KM, et al.
Nucleic Acids Research, 34(11), 3288-3288 (2006)
Gohei Nishibuchi et al.
The Journal of biological chemistry, 289(42), 28956-28970 (2014-09-06)
Histone H3K4 methylation has been linked to transcriptional activation. KDM5A (also known as RBP2 or JARID1A), a member of the KDM5 protein family, is an H3K4 demethylase, previously implicated in the regulation of transcription and differentiation. Here, we show that
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