推荐产品
生物来源
rat aorta (adult)
包装
pkg of 500,000 cells
制造商/商品名称
Cell Applications, Inc
生长模式
Adherent
形态学
Endothelial
技术
cell culture | mammalian: suitable
相关疾病
diabetes; cardiovascular diseases
储存温度
−196°C
相关类别
一般描述
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
RAOEC from Cell Applications, Inc. provide a useful model system to study many aspects of cardiovascular function and disease. Co-culture of the artery endothelial cells with species-matched smooth muscle cells provides an ideal model for studying the interaction between these two cell types.
RAOEC from Cell Applications, Inc. have been utilized in a number of research publications to:
Additionally, RAOEC from Cell Applications, Inc. were used to investigate the reasons for differential effects of Intermedin (IMD) on permeability of endothelial cells from different vascular beds (Aslam, 2011). Specifically, it was shown that IMD increased permeability of rat coronary microvascular endothelial cells by inducing loss of VE-cadherin from cel-cell junctions, but it reduced permeability of RAOEC and HUVECs because in those cell types IMD instead caused accumulation of VE-cadherin in cell-cell junctions. These differential effects of IMD on VE-cadherin are explained by the fact that, although IMD activates cAMP/PKA and inhibits RhoA/ROCK pathways leading to rearrangement of actin in both cell types, it only causes downstream Rac1 inhibition in rat coronary microvascular endothelial cells, but not in RAOEC or HUVEC. These results highlight the fundamental differences between endothelial cells from different vascular beds and/or microvascular vs. macrovascular endothelia.
RAOEC from Cell Applications, Inc. provide a useful model system to study many aspects of cardiovascular function and disease. Co-culture of the artery endothelial cells with species-matched smooth muscle cells provides an ideal model for studying the interaction between these two cell types.
RAOEC from Cell Applications, Inc. have been utilized in a number of research publications to:
- Investigate critical signaling pathways and mechanisms relevant to proper endothelial function, such as angiogenesis, proliferation, permeability, and search for beneficial modulators for therapeutic use (Sorensen, 2008; Liu, 2009; Makino, 2009; Masuda, 2012; Gros, 2013; Nguen, 2013)
- Elucidate molecular mechanisms of various cardiovascular risk factors, including those associated with diabetes, polycystic kidney disease, treatment for allograft rejection and vascular ER stress (Makino, 2008; Chiasson, 2011, Ren, 2011; Padilla, 2013)
- Provide a gold standard control for endothelial markers vWF and PECAM/CD31 expression (Hanley, 2008; Imamura, 2010)
- Develop drug delivery system for thrombolytic treatment (Mei, 2010)and methods for cardiac regeneration (Lionetti, 2010); design better vascular implants (Ibrahim, 2008; Yao, 2008; Duffy, 2011) and bone tissue engineering (Hamid, 2014)
Additionally, RAOEC from Cell Applications, Inc. were used to investigate the reasons for differential effects of Intermedin (IMD) on permeability of endothelial cells from different vascular beds (Aslam, 2011). Specifically, it was shown that IMD increased permeability of rat coronary microvascular endothelial cells by inducing loss of VE-cadherin from cel-cell junctions, but it reduced permeability of RAOEC and HUVECs because in those cell types IMD instead caused accumulation of VE-cadherin in cell-cell junctions. These differential effects of IMD on VE-cadherin are explained by the fact that, although IMD activates cAMP/PKA and inhibits RhoA/ROCK pathways leading to rearrangement of actin in both cell types, it only causes downstream Rac1 inhibition in rat coronary microvascular endothelial cells, but not in RAOEC or HUVEC. These results highlight the fundamental differences between endothelial cells from different vascular beds and/or microvascular vs. macrovascular endothelia.
细胞系来源
Aorta
应用
Cardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testing.
组分
Rat Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO
质量
Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
制备说明
- 3rd passage, >500,000 cells in Rat Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least to 6th passage
传代培养常规
Please refer to the RAOEC Culture Protocol.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Geoffrey de Couto et al.
American journal of physiology. Heart and circulatory physiology, 323(5), H892-H903 (2022-09-10)
Heart failure with preserved ejection fraction (HFpEF) is defined by increased left ventricular (LV) stiffness, impaired vascular compliance, and fibrosis. Although systemic inflammation, driven by comorbidities, has been proposed to play a key role, the precise pathogenesis remains elusive. To
Mikhail A Kaplan et al.
Journal of biomaterials science. Polymer edition, 31(11), 1405-1420 (2020-04-24)
The novelty of the work lies in the creation and study of the physical and biological properties of biodegradable polymer coatings for stents based on poly(lactic-co-glycolic acid) (PLGA). Polymer coatings are capable of prolonged and directed release of molecules with
Elise C Wilcox et al.
Frontiers in immunology, 13, 946794-946794 (2022-08-26)
Endothelial cells (ECs) are central to vascular health but also interact with and regulate the immune system. Changes in endothelial state enable immune cells to migrate into the tissue to facilitate repair and fight infection. ECs modulate the function of
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