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Merck
CN

R1780

Sigma-Aldrich

RPMI-1640 培养基

With ʟ-glutamine, sodium bicarbonate without arginine, leucine, lysine, and phenol red, sterile liquid, suitable for cell culture, suitable for isotope labeling for cell culture applications

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别名:
Roswell Park Memorial Institute 1640 medium
MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

NaHCO3: 2 g/L
phenol red: no
glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L

运输

ambient

储存温度

2-8°C

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一般描述

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。

重悬

设计用于细胞培养应用中氨基酸稳定同位素标记。
补充 0.100 g/L L -精氨酸- 13 C 6 15 N 4 HCl(产品 608033)、0.100 g/L L -亮氨酸- 13 C 6 15 N(产品 608068)和 0.100 g/L L -赖氨酸- 13 C 6 15 N 2(产品 608041)。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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