R128
Monoclonal Anti-Ryanodine Receptor antibody produced in mouse
clone C3-33, ascites fluid
别名:
Anti-CCO, Anti-KDS, Anti-MHS, Anti-MHS1, Anti-PPP1R137, Anti-RYDR, Anti-RYR, Anti-RYR-1, Anti-SKRR
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关于此项目
Conjugate:
unconjugated
Clone:
C3-33, monoclonal
Application:
immunohistochemistry (frozen sections)
immunoprecipitation (IP)
western blot (chemiluminescent)
immunoprecipitation (IP)
western blot (chemiluminescent)
Species reactivity:
canine, rabbit, rat, chicken, amphibian, guinea pig, mouse, fish, human
Citations:
11
Technique(s):
immunohistochemistry (frozen sections): 1 μg/mL using 4% paraformaldehyde-fixed, frozen mouse brain tissue
immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1 μg/mL
immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1 μg/mL
Uniprot accession no.:
产品名称
Monoclonal Anti-Ryanodine Receptor antibody produced in mouse, clone C3-33, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
C3-33, monoclonal
mol wt
antigen ~565 kDa (non-mammalian vertebrates, a doublet at 565 kDa represents the α and β isoforms)
species reactivity
canine, rabbit, rat, chicken, amphibian, guinea pig, mouse, fish, human
concentration
~1 mg/mL
technique(s)
immunohistochemistry (frozen sections): 1 μg/mL using 4% paraformaldehyde-fixed, frozen mouse brain tissue
immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1 μg/mL
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
rat ... Ryr1(114207), Ryr2(84025)
Application
Anti-Ryanodine Receptor has been used in immunofluorescence assay.
Biochem/physiol Actions
In rats PASMCs (pulmonary artery smooth muscle cells), RyR2 (ryanodine receptor isoform 1) participates in RyR-gated Ca2+ entry. It controls the secretion of insulin and glucose homeostasis. After hemorrhagic shock, RyR2 involves in the progress of vascular bi-phasic reactivity to NE (norepinephrine). In human, RyR1 plays an important role in muscle contraction. RYR1 mutation causes CCD (central core disease) and MHS (malignant hyperthermia susceptibility).
The antibody reacts strongly with RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform), and weakly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform). It reacts with ryanodine receptor in cardiac muscle of canine, rat, finch and pigeon; in visceral smooth muscle of toad; and in rat brain. It reacts with skeletal muscle of fish and the β isoform of frog, but only weakly with skeletal muscle in rabbit and the α isoform of frog. In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in the hippocampus.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
RyR1 (ryanodine receptor isoform 1) is a homotetrameric intracellular calcium channel, that is present in skeletal muscle tissue. It is located on human chromosome 19q13.1. In rats, RyR1 and RyR2 are expressed in PASMCs (pulmonary artery smooth muscle cells).
Immunogen
dog cardiac ryanodine receptors (RyR)
Physical form
Containing PBS and 0.05% sodium azide
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Imaging Ca2+ nanosparks in heart with a new targeted biosensor
Shang W, et al.
Circulation Research, 114(3), 412-420 (2014)
Wei Shang et al.
Circulation research, 114(3), 412-420 (2013-11-22)
In cardiac dyads, junctional Ca2+ directly controls the gating of the ryanodine receptors (RyRs), and is itself dominated by RyR-mediated Ca2+ release from the sarcoplasmic reticulum. Existing probes do not report such local Ca2+ signals because of probe diffusion, so
Glucose-Dependent Insulin Secretion in Pancreatic ?-Cell Islets from Male Rats Requires Ca2+ Release via ROS-Stimulated Ryanodine Receptors
Llanos P, et al.
PLoS ONE, 10(6) (2015)
Conformation of ryanodine receptor-2 gates store-operated calcium entry in rat pulmonary arterial myocytes
Lin AH, et al.
Cardiovascular Research, 111(1), 94-104 (2016)
An autosomal dominant congenital myopathy with cores and rods is associated with a neomutation in the RYR1 gene encoding the skeletal muscle ryanodine receptor
Monnier N, et al.
Human Molecular Genetics, 9(18), 2599-2608 (2000)
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