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等级
for molecular biology
表单
buffered aqueous solution
浓度
10000-50000 units/mL
UniProt登记号
异质活性
DNase, RNase, protease, none detected
nonspecific endonuclease, absence of activity (after 20 hour incubation)
储存温度
−20°C
基因信息
bacteriophage SP6 ... SP6p08(1481778)
一般描述
Sp6 Polymerase is a DNA-dependent RNA polymerase with high specific activity for the bacteriophage Sp6 promoter. The enzyme is efficient in transcribing large quantities of RNA from the DNA sequence (polylinker) downstream from the promoter. Simultaneous transcription by Sp6 and Sp7 polymerases may occur without interfering with each other.
应用
Suitable for:
- Synthesis of labeled and unlabeled RNA transcripts
- RNase protection assays
- Generating RNA transcripts for in vitro translation
- Use in in vitro transcription of RNA transcripts, antisense RNA (from reversed cloned DNA insert), and biologically active mRNA
组分
Protease is supplied in a solution of 100 mM NaCl, 50 mM Tris-HCl (pH 7.9), 0.1 mM EDTA, 0.1% Triton X-100, 1 mM DTT, and 50% (v/v) glycerol.
单位定义
One unit will catalyze the incorporation of 1 nmol of rNTP into acid-precipitable material in 60 min at 37 °C.
分析说明
Activity assay: 40 mM Tris-HCl, pH 7.9, 6 mM MgCl2, 4 mM spermidine, 10 mM DTT, 0.5 μM each rNTP + 10 μCi α-32P-UTP, 3-10 units of enzyme, and 1 μg of a 350 bp template are incubated for 10 min at 37 °C in a total volume of 100 μl. Typical results are ≥50% incorporation of labeled nucleotide into ≥90% full-length transcript.
其他说明
Sp6 polymerase exhibits 30% more activity at 40°C than at 37°C.
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说明
价格
警示用语：
Warning
危险声明
危险分类
Aquatic Chronic 3 - Eye Irrit. 2
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 18-18 (1989)
M R Green et al.
Cell, 32(3), 681-694 (1983-03-01)
To study the mechanisms of RNA splicing we have synthesized beta-globin mRNA precursors by in vitro transcription using a plasmid in which a human beta-globin gene is fused to an efficient bacteriophage promoter. The structural requirements for accurate splicing of
E T Schenborn et al.
Nucleic acids research, 13(17), 6223-6236 (1985-09-11)
The in vitro synthesis of extraneous RNA sequences by SP6 and T7 RNA polymerases from specific DNA templates is described. Transcription of templates prepared by digestion with restriction enzymes that leave 3' protruding ends resulted in the production of significant
D A Melton et al.
Nucleic acids research, 12(18), 7035-7056 (1984-09-25)
A simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure is described. This in vitro transcription system is based on the unusually specific RNA synthesis by bacteriophage SP6 RNA polymerase which initiates transcription exclusively at
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