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Merck
CN

R0155

Anti-Anti-RanGAP1 (C-terminal) 兔抗

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-Ran-GTPase-Activating Protein 1

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD09265371

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产品名称

Anti-Anti-RanGAP1 (C-terminal) 兔抗, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~65 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using HeLa cells
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using HEK-293T cells transfected with human RanGAP1

UniProt accession no.

P46060

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RANGAP1(5905)
mouse ... Rangap1(19387)

Physical form

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

Application

Anti-RanGAP1 (C-terminal) antibody produced in rabbit has been used for immunoblotting and listeriolysin O (LLO) purification.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Biochem/physiol Actions

RanGAP1 (Ran GTPase Activating Protein 1, 65 kDa) is a key regulator of Ran activity, specifically inducing the GTPase activity of Ran. RanGAP1 is conjugated to the small ubiquitin-related modifier protein SUMO-1. The association of RanGAP1 with RanBP2 facilitates nuclear transport.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The unmodified form of RanGAP1 (65 kDa) is exclusively cytoplasmic, whereas the 90 kDa SUMO-1-modified form is associated with the cytoplasmic fibers of the nuclear pore complex (NPC).

Immunogen

synthetic peptide corresponding to amino acids 573-587 located at the C-terminus of human RanGAP1. The sequence is highly conserved (80% identity) in mouse RanGAP1.

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存储类别

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

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分析证书(COA)

Lot/Batch Number
068M4898V
027K4799

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Listeria monocytogenes impairs SUMOylation for efficient infection
Ribet D, et al.
Nature, 464(7292), 1192-1192 (2010)
The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo
Joseph J, et al.
Current Biology, 14(7), 611-617 (2004)
David Ribet et al.
Nature, 464(7292), 1192-1195 (2010-04-24)
During infection, pathogenic bacteria manipulate the host cell in various ways to allow their own replication, propagation and escape from host immune responses. Post-translational modifications are unique mechanisms that allow cells to rapidly, locally and specifically modify activity or interactions
Smriti Verma et al.
Molecular and cellular biology, 35(17), 2932-2946 (2015-06-24)
Posttranslational modifications (PTMs) can alter many fundamental properties of a protein. One or combinations of them have been known to regulate the dynamics of many cellular pathways and consequently regulate all vital processes. Understandably, pathogens have evolved sophisticated strategies to

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