生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~65 kDa
种属反应性
human
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
浓度
~1 mg/mL
技术
indirect immunofluorescence: 5-10 μg/mL using HeLa cells
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using HEK-293T cells transfected with human RanGAP1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... RANGAP1(5905)
mouse ... Rangap1(19387)
一般描述
The unmodified form of RanGAP1 (65 kDa) is exclusively cytoplasmic, whereas the 90 kDa SUMO-1-modified form is associated with the cytoplasmic fibers of the nuclear pore complex (NPC).
免疫原
synthetic peptide corresponding to amino acids 573-587 located at the C-terminus of human RanGAP1. The sequence is highly conserved (80% identity) in mouse RanGAP1.
应用
Anti-RanGAP1 (C-terminal) antibody produced in rabbit has been used for immunoblotting and listeriolysin O (LLO) purification.
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.
生化/生理作用
RanGAP1 (Ran GTPase Activating Protein 1, 65 kDa) is a key regulator of Ran activity, specifically inducing the GTPase activity of Ran. RanGAP1 is conjugated to the small ubiquitin-related modifier protein SUMO-1. The association of RanGAP1 with RanBP2 facilitates nuclear transport.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Listeria monocytogenes impairs SUMOylation for efficient infection
Ribet D, et al.
Nature, 464(7292), 1192-1192 (2010)
The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo
Joseph J, et al.
Current Biology, 14(7), 611-617 (2004)
David Ribet et al.
Nature, 464(7292), 1192-1195 (2010-04-24)
During infection, pathogenic bacteria manipulate the host cell in various ways to allow their own replication, propagation and escape from host immune responses. Post-translational modifications are unique mechanisms that allow cells to rapidly, locally and specifically modify activity or interactions
Salmonella Engages Host MicroRNAs To Modulate SUMOylation: a New Arsenal for Intracellular Survival.
Smriti Verma et al.
Molecular and cellular biology, 35(17), 2932-2946 (2015-06-24)
Posttranslational modifications (PTMs) can alter many fundamental properties of a protein. One or combinations of them have been known to regulate the dynamics of many cellular pathways and consequently regulate all vital processes. Understandably, pathogens have evolved sophisticated strategies to
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