推荐产品
用途
sufficient for 10 purifications
运输
wet ice
储存温度
2-8°C
一般描述
蛋白A抗体纯化试剂盒为分离单克隆和多克隆抗体提供所有所需试剂。
特异性
容量/柱（按物种分类）
人IgG 20-25 mg
小鼠IgG1 8-12 mg
小鼠IgG2a 10-15 mg
小鼠IgG2b 6-10 mg
小鼠IgG3 15-20 mg
兔IgG 8-12 mg
山羊IgG 2-4 mg
牛IgG 8-12 mg
人IgG 20-25 mg
小鼠IgG1 8-12 mg
小鼠IgG2a 10-15 mg
小鼠IgG2b 6-10 mg
小鼠IgG3 15-20 mg
兔IgG 8-12 mg
山羊IgG 2-4 mg
牛IgG 8-12 mg
应用
PURE-1A以预先包装、易于使用的试剂盒形式提供蛋白A技术。使用该试剂盒，可以从血清、腹水或细胞培养物上清液中纯化出毫克量的IgG。
可能可以从其他物种纯化IgG，但是，研究人员必须确定该试剂盒对其应用的适用性。
蛋白A抗体纯化试剂盒适用于纯化人、小鼠、兔、山羊和牛的IgG。
特点和优势
- 高容量 - 每柱纯化多达8 mg小鼠IgG或25 mg人IgG
- 特异性 - 仅结合IgG
- 易于使用 - 仅需一步即可完成抗体洗脱和脱盐，随时可用
- 温和 - 避免长时间暴露于低pH值的抗体
原理
使用我们的柱式系统，可以在生理pH下将抗体作为高度纯化的蛋白质洗脱。蛋白A是从哺乳动物宿主中分离抗体的有力工具。蛋白A对IgG具有高度特异性，并确保抗体制剂几乎不含IgA、IgM和非免疫球蛋白血清蛋白，如白蛋白。
仅试剂盒组分
产品编号
说明
- Binding Buffer 225 mL
- Desalting Cartridge 1 ea
- Elution Buffer 75 mL
- HEPES Buffer 225 mL
- 5 ml Syringe 1
- 10 ml Syringe 1
- Protein A Cartridge 1 mL
- Regeneration Buffer 75 mL
查看所有结果 (8)
警示用语：
Danger
危险声明
危险分类
Acute Tox. 4 Oral - STOT SE 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Andreas H Laustsen et al.
Toxicon : official journal of the International Society on Toxinology, 99, 23-35 (2015-03-17)
The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identified or assigned to families. Estimation of relative protein abundances revealed that venom is
Ignacio Gallardo-Carreño et al.
Frontiers in microbiology, 11, 590736-590736 (2021-01-05)
Perchlorate anions are produced by chemical industries and are important contaminants in certain natural ecosystems. Perchlorate also occurs in some natural and uncontaminated environments such as the Atacama Desert, the high Arctic or the Antarctic Dry Valleys, and is especially
Penio T Todorov et al.
Cancer research, 67(23), 11419-11427 (2007-12-07)
Proteolysis-inducing factor (PIF) is a sulfated glycoprotein produced by cachexia-inducing tumors, which induces atrophy of skeletal muscle. PIF has been shown to bind specifically with high affinity (K(d), in nanomolar) to sarcolemma membranes from skeletal muscle of both the mouse
Improved ELISA test for determination of potency of Inactivated Poliovirus Vaccine (IPV)
Hwang,G et al.
Sensors, 8(12), 2074-2079 (2008)
Marcella de Champdoré et al.
Analytical chemistry, 79(2), 751-757 (2007-01-16)
Patulin is a toxic secondary metabolite of a number of fungal species belonging to the genera Penicillum and Aspergillus. It has been mainly isolated from apples and apple products contaminated with the common storage-rot fungus of apples, Penicillum expansum, but
实验方案
Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门