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用途
sufficient for 96 reactions
运输
wet ice
储存温度
2-8°C
分析说明
Detection and characterization of protein tyrosine phosphatase (PTP) activity during purification (column fractions, etc.) of natural and recombinant PTPases, such as LAR phosphatase (CD45), in cell, tissue extracts and immunoprecipitates. The kit rapidly detects the inorganic phosphate released by PTPase from synthetic phosphotyrosine peptide substrates based on a colorimetric assay using malachite green-molybdate complex that shifts in light absorbance to 620-630 nm in the presence of inorganic phosphates.
附注
Phosphotyrosine substrates supplied are sufficient for 120 reactions.
仅试剂盒组分
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说明
- Dye Additive Solution 1 mL
- Malachite Green-Molybdate Dye Solution 1 x 25
- Phosphate Standard (10 mM) 1 mL
- Phosphotyrosine Peptide Substrate 2, DADEpYLIPQQG 1.6 mg
- Phosphotyrosine Peptide Substrate 1, TRDIpYETDYYRK 1.7 mg
- Sephadex G-25 Prepacked Column (3 ml) 5 ea
- Water, phosphate-free 25 mL
- 96 Well Microtiter Plate A1/2 area with cover 1 ea
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