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文件

安全信息

PRS2265

Sigma-Aldrich

抗 Nephrin 兔抗

affinity isolated antibody, buffered aqueous solution

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别名:
CNF, NPHN, 肾小球特异性细胞粘附受体
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

rabbit

质量水平

100

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

形式

buffered aqueous solution

种属反应性

rat, human, mouse

技术

immunofluorescence: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

NCBI登记号

NP_004637

UniProt登记号

O60500

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... NPHS1(4868)

一般描述

肾病蛋白（nephrin）在肾小球中强烈表达，是细胞粘附分子免疫球蛋白家族的成员之一。它是一种1241个残基的跨膜蛋白。Nephrin基因的突变会导致先天性肾病综合征，这是一种常染色体隐性遗传疾病，其特征是子宫内有大量蛋白尿和出生时的肾病。肾小球允许正常肾脏过滤血浆，使其非常纯净。Nephrin在肾小球的足细胞裂孔隔膜中表达，其方式表明Nephrin分子以类似于粘附连接中的钙黏着蛋白各种相互作用的反平行方式同型二聚化。因此，肾素可以构成裂孔隔膜的整个细胞外结构。

免疫原

肾蛋白抗体在人类的羧基末端附近产生抗14个氨基酸的合成肽的抗体
肾蛋白。免疫原位于肾蛋白的最后 50 个氨基酸内。

联系

可以使用阻断肽 SBP2265阻断该抗体的作用。

外形

溶于含0.02%叠氮化钠的磷酸盐缓冲盐水中

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Why does the Nephrin from mouse kidney indicate a molecular weight of a little less than 100 kDa on the Western blot shown on the webpage for Product PRS2265, anti-Nephrin antibody produced in rabbit?

    Based on the literature, one would expect the Nephrin to show up at a higher molecular weight. Unfortunately, we do not have a good explanation for the mouse Nephrin band showing up at a molecular weight of a little less than 100 kDa. It might be due to a cleavage event, but we have not read of that happening with Nephrin. The antibody may be detecting an alternative splicing product, but again, we have not seen anything like that mentioned in the literature on Nephrin.The sequence used as the antigen for this antibody does not possess homology to any other protein. And the PRS2265 antibody is quite specific for this sequence, based on the results when using a blocking peptide as shown in lane B of the blot: Supplier is confident that the antibody is detecting Nephrin but is not able to be of more help in explaining the lower-than-expected molecular weight.According to the Uniprot link for mouse nephrin, the mature form of mouse Nephrin has been described as a protein that is a single polypeptide of 1221 amino acids (1256 minus the signal peptide of 35 amino acids)So based on the amino acids alone, one would expect a band close to 130 kDa.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Vladislav E Sobolev et al.
International journal of molecular sciences, 22(24) (2021-12-25)
The delayed effects of acute intoxication by organophosphates (OPs) are poorly understood, and the various experimental animal models often do not take into account species characteristics. The principal biochemical feature of rodents is the presence of carboxylesterase in blood plasma
Positionally Cloned Gene for a Novel Glomerular Protein?Nephrin?Is Mutated in Congenital Nephrotic Syndrome
Marjo Kestilla
Molecular Cell, 1(4), 575-582 (1998)
Balajikarthick Subramanian et al.
Kidney international, 90(2), 363-372 (2016-06-29)
Mutations in the INF2 (inverted formin 2) gene, encoding a diaphanous formin family protein that regulates actin cytoskeleton dynamics, cause human focal segmental glomerulosclerosis (FSGS). INF2 interacts directly with certain other mammalian diaphanous formin proteins (mDia) that function as RhoA
Dong Wang et al.
International journal of molecular sciences, 19(1) (2018-01-06)
Obesity and obesity related kidney and liver disease have become more prevalent over the past few decades, especially in the western world. Sodium-glucose cotransporter 2 (SGLT2) inhibitors are a new class of antidiabetic agents with promising effects on cardiovascular and
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