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Merck
CN

PROT20LC

Millipore

ProteoPrep® 20 等离子免疫耗尽液相色谱柱

别名:

ProteoPrep® 20

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About This Item

UNSPSC代码:
12352200

储存温度

2-8°C

一般描述

用ProteoPrep20 LC色谱柱更快发现蛋白生物标志物! ProteoPrep20 LC色谱柱从人血浆或血清中去除了20种高度丰富的蛋白质(占蛋白质组的97%),揭示了先前掩盖的生物学上重要的生物标志物。 这二十种蛋白质的平均效率为98%(白蛋白和IgG为>99%,其余18种蛋白质为>85%)。 然后可以将耗尽的,具有生物学意义的剩余蛋白质浓缩至少20倍。

ProteoPrep20 LC色谱柱可容纳100微升的血浆或血清样品,并将消耗100个或更多的样品。 这有效地实现了10,000微升血浆或血清的免疫消耗。

ProteoPrep20 LC色谱柱设计用于低压或中压液相色谱系统。只要对系统进行较小的改动,它也可以与高压液相色谱系统一起使用。 如需了解与HPLC系统一起使用ProteoPrep20 Lc色谱柱的更多信息,请查阅用户指南。

ProteoPrep20 LC色谱柱可特异性消耗以下血浆蛋白:
白蛋白
IgG
转铁蛋白
纤维蛋白原
IgA
α2-巨球蛋白
IgM
α1-抗胰蛋白酶
补体C3
结合球蛋白
载脂蛋白A1
载脂蛋白A2
载脂蛋白B
α1-酸性糖蛋白
铜蓝蛋白
补体C4
补体C1q
IgD
前白蛋白
纤溶酶原

应用

Proteoprep 20是一种免疫耗竭液相色谱(LC)色谱柱,可从血浆或血清中去除20种丰富的干扰蛋白,从而可以分析较不丰富的蛋白。Proteoprep 20去除:白蛋白,IgG,转铁蛋白,纤维蛋白原,IgA,α2-微球蛋白,IgM,α1-抗胰蛋白酶,补体C3,触珠蛋白,载脂蛋白A1,A3和B;α1-酸性糖蛋白、铜蓝蛋白、补体C4、C1q;IgD,前白蛋白和纤溶酶原。

特点和优势

亲自探索ProteoPrep20 LC色谱柱!
  • 通过去除20种高度丰富的蛋白质来降低样品的复杂性并提高效率
  • 揭示先前被掩盖的蛋白质
  • 富集目标蛋白达20倍

法律信息

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • CLS8160Corning® Costar® Spin-X® centrifuge tube filters, cellulose acetate membrane, pore size 0.22 μm, sterile化学品安全说明书

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

历史批次信息供参考:

分析证书(COA)

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访问文档库

Tatiana Plavina et al.
Journal of proteome research, 6(2), 662-671 (2007-02-03)
We report on the development of a robust and relatively high-throughput method for in-depth proteomic analysis of human plasma suitable for biomarker discovery. The method consists of depletion of albumin and IgG and multi-lectin affinity chromatography (M-LAC), followed by nanoLC-MS/MS
Thomas Linke et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 849(1-2), 273-281 (2006-12-26)
The proteomic analysis of plasma and serum samples represents a formidable challenge due to the presence of a few highly abundant proteins such as albumin and immunoglobulins. Detection of low abundance protein biomarkers requires therefore either the specific depletion of
M K Disni R Dayarathna et al.
Journal of separation science, 31(6-7), 1156-1166 (2008-02-29)
Plasma is an important biological material for biomarker discovery. However, the wide dynamic range in protein concentration remains a major challenge. In this paper, we introduce the development of a proteomic platform for analysis of plasma samples. The method utilizes
Nicholas A Cellar et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 877(1-2), 79-85 (2008-11-29)
Proteomic analysis can be hampered by the large concentration distribution of proteins. Immunoaffinity techniques have been applied to selectively remove high abundant proteins (HAP's) from samples prior to analysis. Although immunodepletion of HAP's has been shown to enable greater detection
Nitin Seam et al.
Clinical chemistry, 53(11), 1915-1920 (2007-09-25)
Prefractionation techniques such as serum albumin depletion are useful precursors to proteomic analysis, but they may introduce preanalytical bias if the depletion is not reproducible. We examined the reproducibility of albumin immunodepletion and describe a method of QC for this

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