PPC1010
蛋白酶和磷酸酶抑制剂混合物
DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase
别名:
蛋白酶和磷酸酶抑制剂混合物
登录 查看组织和合同定价。
选择尺寸
关于此项目
UNSPSC Code:
12352200
NACRES:
NA.54
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C
产品名称
蛋白酶和磷酸酶抑制剂混合物,
form
DMSO solution
packaging
pkg of 1 ml, 5 ml ×
solubility
water: soluble
shipped in
wet ice
storage temp.
−20°C
Quality Level
Application
蛋白酶和磷酸酶抑制剂混合物可用作放射性免疫沉淀分析(RIPA)缓冲液：
- 用于细胞蛋白质提取
- 裂解纯化的CD4+ T细胞
- 制备胎盘组织和细胞的蛋白提取物
蛋白酶和磷酸酶抑制剂鸡尾酒已用于蛋白质分离缓冲液。
General description
蛋白酶和磷酸酶抑制剂混合物，设计用于增加细胞提取物中完整和未修饰蛋白质的产量。蛋白酶和磷酸酶抑制剂混合物抑制丝氨酸、半胱氨酸和酸性蛋白酶，以及氨肽酶。 它还抑制碱性磷酸酶的丝氨酸/苏氨酸蛋白磷酸酶和L-同工酶。 该混合物已经在各种动物组织（例如人胎盘和牛肝）的提取物，以及 A431、CHO和U937细胞的提取物上进行了测试。蛋白酶和磷酸酶抑制剂鸡尾酒以溶于DMSO的即用型溶液提供。
Other Notes
本产品仅供研&发使用，不得用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。
Preparation Note
生物提取物中混合物的推荐稀释度为从组织或细胞中提取的每 500mg蛋白质的1ml 混合物。在许多情况下，混合物可以以 1％（v/v）的终浓度使用，每 100ml 提取缓冲液使用1ml鸡尾酒溶液。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F
flash_point_c
87 °C
法规信息
高风险级别生物产品--毒素类产品
此项目有
Marcela Rassi-Cruz et al.
Endocrine-related cancer, 28(1), 1-13 (2020-10-29)
Familial primary aldosteronism (PA) is rare and mostly diagnosed in early-onset hypertension (HT). However, 'sporadic' bilateral adrenal hyperplasia (BAH) is the most frequent cause of PA and remains without genetic etiology in most cases. Our aim was to investigate new
E Zoico et al.
Mechanisms of ageing and development, 192, 111374-111374 (2020-10-06)
Fibrosis has been considered as a hallmark of dysfunctional adipose tissue (AT), however the role and mechanisms of fibrosis in the age related AT dysfunction are not yet well characterized. The aim of the study was to investigate the mechanisms
LKB1-PTEN axis controls Th1 and Th17 cell differentiation via regulating mTORC1
Pandit M, et al.
Journal of Molecular Medicine, 99(8), 1139-1150 (2021)
miR-433 suppresses tumor progression via Smad2 in non-small cell lung cancer
Li J, et al.
Pathology Research and Practice, 215(10), 152591-152591 (2019)
Ramaian Santhaseela Anand et al.
Cell stress & chaperones, 25(6), 945-953 (2020-06-24)
Structures of cellular organelles are intertwined with their functions that undergo alterations once the organelles are stressed. Since organelle functions are dependent on each other, an organelle-specific stress possibly influences the structure and function of its associated organelles. In this
相关内容
细胞裂解和蛋白质溶解是进行有效分析和高效处理的关键步骤，默克分享提取方法包括酶法、化学法、机械法或几种方法的联合使用。
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
Instructions
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持