一般描述
GlycoProfile™labeling kit专门设计用于高效地使用2-氨基苯甲酰胺（2-AB）或2-氨基苯甲酸（氨茴酸；2-AA）来对N-连接、O-连接、及糖基磷脂酰肌醇（GPI）锚定的多糖进行标记。这些小荧光素可增强多糖的光谱吸收，提高HPAE（高性能阴离子交换色谱）、HPLC、及ESI-MS（电喷射质谱）等方法标记多糖的检测效果。其结合非常可靠，所得的染料-多糖轭合物也很稳定，在标记后的分析过程中不会发生降解。
应用
GlycoProfile™ 2-AA Labeling Kit has been used in the labeling of fluorescently derivatized N-glycans from chinese hamster ovary CHO cells.
While 2-AB is slightly less sensitive than 2-AA, it is suitable for downstream glycan analysis by HPAE, HPLC and ESI-MS. Although suitable for the same applications, 2-AA is better suited for SDS-PAGE.
法律信息
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
仅试剂盒组分
产品编号
说明
- Anthranilic acid (2-AA) 2 x 6
- Dimethyl sulfoxide (DMSO) 2 x 350
- Acetic acid, glacial 2 x 200
- Reductant (sodium cyanoborohydride) 2 x 6
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1
WGK
WGK 1
闪点(°F)
302.0 °F
闪点(°C)
150 °C
法规信息
监管及禁止进口产品
Weiguo Fang et al.
Plant physiology, 154(3), 1549-1557 (2010-09-15)
The symbiotic associations between rhizospheric fungi and plants have enormous environmental impact. Fungi are crucial to plant health as antagonists of pathogens and herbivores and facilitate the uptake of soil nutrients. However, little is known about the plant products obtained
Modulation of IgG1 immunoeffector function by glycoengineering of the GDP-fucose biosynthesis pathway
Kelly RM, et al.
Biotechnology and Bioengineering, 115(3), 705-718 (2018)
商品
Glycoprofile Labeling Kits efficiently label glycans for glycan analysis, offering flexibility in choice of labeling.
蛋白质的结构修饰对于活细胞是必不可少的。发生调节异常时，它们往往是会引发疾病。聚糖是生物系统中许多结构变异的原因，它们在细胞表面的表现通常被称为“糖”。
Glycans play a key role in protein structure and disease; representation on cell surfaces is the glycome.
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