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Merck
CN

PP0100

Trypsin Profile IGD Kit

别名:

In gel digestion kit, Proteomics grade Trypsin, TPCK treated

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UNSPSC代码:
12352200
NACRES:
NA.56

主要文件

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用途

 kit sufficient for ≤100 applications

质量水平

200

储存温度

2-8°C

相关类别

一般描述

Trypsin Profile IGD Kit 能够对多达 100 个切除的蛋白点进行快速、高效和完全的凝胶内胰蛋白酶消化。消化后的蛋白质可用于 MALDI-MS（基质辅助激光解吸/电离质谱法），无需额外制备。由于 Trypsin Profile IGD Kit 含有蛋白质组学级别的胰蛋白酶，在 MALDI 光谱中观察到较高的序列覆盖率和较少的模棱两可的自溶峰。
通过赖氨酸的ε-氨基的还原甲基化对胰蛋白酶进行化学修饰，减少自溶，使自溶片段最小化。此外，还经过 TPCK（对甲苯磺酰基苯丙氨酰基氯甲基酮）处理，去除残留的糜蛋白酶活性，然后通过亲和层析进一步纯化，得到了适合蛋白质组学工作的高纯度胰蛋白酶。

应用

胰蛋白酶谱 IGD 试剂盒已用于 LC-MS（液相色谱-质谱法）/MS 和 MALDI-TOF（基质辅助激光解吸电离-飞行时间）质谱分析的凝胶内消化。

特点和优势

  • 相比其它凝胶中消化试剂盒来说可更快速地完成脱色
  • 包含对感兴趣蛋白/多肽进行脱色、消化及提取所需的所有试剂
  • 兼容使用Coomassie, SYPRO™ Orange, SYPRO Ruby, and ProteoSilver™等进行染色的凝胶
  • 所得样品可即用于MALDI-MS或HPLC-MS分析

法律信息

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC
SYPRO is a trademark of Thermo-Fisher Scientific, Inc.

仅试剂盒组分

产品编号
说明
  • Destaining Solution Reconstituted to 75

警示用语：

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

3 - Flammable liquids

闪点(°F)

35.6 °F - closed cup

闪点(°C)

2.0 °C - closed cup

法规信息

危险化学品
低风险生物材料
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCQ8498
SLCJ8802
SLCD4484
SLCB1308
SLBW2626

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