Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified

Replication protein A2 (RPA32) is encoded by the gene mapped to human chromosome 1p35.3. It is a subunit of the heterotrimeric replication protein A (RPA) complex.

Immunogen was a dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.1 (GeneID 6118).

Replication Protein A (RPA) binds single-stranded DNA and plays a vital role in DNA replication, DNA repair, and checkpoint signaling. Phosphorylation of replication protein A2 (RPA32) by ataxia telangiectasia and Rad3-related protein kinase (ATR) and DNA-dependent protein kinase (DNA-PK) causes checkpoint kinase 1 (Chk1) activation and replication arrest. DNA-PK and ATR signaling via RPA32 (S4/S8) enhances genome stability and cell survival following replication stress.

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.

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