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Merck
CN

PIC0004

Protease Inhibitor Cocktail

ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use in purification of Histidine-tagged proteins, Non-freezing solution

别名:

Protease inhibitor cocktail

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关于此项目

UNSPSC Code:
12352200
NACRES:
NA.28
Form:
ready-to-use solution
Solubility:
water: soluble
Storage temp.:
−20°C

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产品名称

ReadyShield®蛋白酶抑制剂Cocktail, for use in purification of Histidine-tagged proteins, Non-freezing solution

description

Purification of Histidine-Tagged Proteins

form

ready-to-use solution

quality

200

packaging

pkg of 1 mL (in amber polypropylene vial)
pkg of 5 mL (in amber polypropylene vial)

solubility

water: soluble

storage temp.

−20°C

Quality Level

100

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Features and Benefits

  • Does not freeze in storage for quick, convenient use.
  • Tailored for use with Histidine fusion tagged proteins.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

General description

This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000351161
0000351161
0000097736
0000095267
0000097733

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商品

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

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