重组
expressed in E. coli
质量水平
检测方案
≥95% (SDS-PAGE)
形式
lyophilized powder
包装
pkg of 1 kit (4 components)
运输
wet ice
储存温度
−20°C
一般描述
Recombinant PURedit Cas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, Streptococcus pyogenes PURedit Cas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
PURedit translational-grade CRISPR reagents are engineered to meet superior manufacturing standards that enable consistent, high-quality performance every time.
PURedit translational-grade CRISPR reagents are engineered to meet superior manufacturing standards that enable consistent, high-quality performance every time.
应用
Functional Genomics; Target Validation; Genome Editing
特点和优势
- High Quality for preclinical and early clinical research
- Highest performance efficiency of any Cas9 variant
- Ready to inject/transfect
包装
pkg of 50 μg (≥ 300 pmol)
pkg of 250 μg (≥ 1500 pmol)
pkg of 250 μg (≥ 1500 pmol)
组分
Each kit consists of:
- one vial of PURedit Cas9 Recombinant Protein
- one vial of PURedit Transfection Buffer
- one vial of Reconstitution Buffer
- one vial of Dilution Buffer
原理
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
重悬
Lyophilized PURedit Cas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.
其他说明
Use our CRISPR Selection Tool to order gRNA
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
法律信息
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
PURedit is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
法规信息
常规特殊物品
实验方案
Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity
可靠的PURedit™ CRISPR合成gRNA和Cas9蛋白可提供行业领先的靶位点切割和特异性
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
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