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Merck
CN
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文件

安全信息

PE0230

Sigma-Aldrich

植物总蛋白提取试剂盒

Suitable for any plant species or tissue

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UNSPSC代码:
41105500
NACRES:
NA.56

质量水平

200

技术

protein extraction: suitable

储存温度

−20°C

一般描述

植物总蛋白抽提试剂盒（Plant Total Protein Extraction Kit）是专为植物生命科学应用而设计的产品，能够从任意植物种类或组织中抽提蛋白定性样本。本方案无需任何超速离心步骤或含水多聚物的两相分离步骤（APTP）。本试剂盒包含两类试剂：一份植物特异性的蛋白酶抑制剂cocktail、一份增加了溶解能力以抽提更多疏水蛋白质的全新离液剂（C0356, 蛋白抽提试剂第4类试剂，Protein Extraction Reagent Type 4). 在植物组织样品顺序蛋白抽提过程中采用新鲜试剂，能洗脱植物组织中的几乎所有蛋白质。蛋白酶抑制剂cocktail是作用于丝氨酸、半胱氨酸、金属蛋白酶、天冬氨酸与氨肽酶等酶类的多种蛋白酶的广谱抑制剂混合物。本品含有胃酶抑素A，4-（2-氨基乙基）苯磺酰氟（AEBSF）中，E-64，亮肽素，1,10-菲咯啉，和苯丁抑制素等多种成分。经验证，cocktail抑制剂混合物能够在抽提过程中高效避免蛋白降解的发生。

在去除了多酚，鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用离液剂重悬。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得定性的分级蛋白样品，用于下游蛋白组学分析。

应用

植物总蛋白提取试剂盒用于分离种子粗蛋白。还用于提取总蛋白。

数量

足够用于从10-250mg的植物组织样品中制备20个单独的提取物

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • C0356Protein Extraction Reagent Type 4 1 bottle化学品安全说明书
  • P9599Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution 1 mL化学品安全说明书

警示用语：

Warning

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2

WGK

WGK 3

闪点(°F)

185.0 °F

闪点(°C)

85 °C

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the expected protein yield when using Product PE0230, Plant Protein Extraction Kit?

    Depending on the tissue used (age, type (leaf, stem, root) health, etc.) one can expect 1-4 mg/mL at about 75-80% of the total protein.

  4. How can I improve my yield when using Product PE0230, Plant Protein Extraction Kit?

    A second extraction can be performed and combined with the first to increase protein extracted to about 95% of the total.

  5. Will my protein samples be denatured when using Product PE0230, Plant Protein Extraction Kit?

    Yes. The chaotropic agents and the detergent in the kit will denature the proteins at least partially.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Revealing proteins associated with symbiotic germination of Gastrodia elata by proteomic analysis
Zeng X, et al.
Botanical studies, 59(1), 8-8 (2018)
Xu Zeng et al.
Botanical studies, 59(1), 8-8 (2018-03-08)
Gastrodia elata, a mycoheterotrophic orchid, is a well-known medicinal herb. In nature, the seed germination of G. elata requires proper fungal association, because of the absence of endosperm. To germinate successfully, G. elata obtains nutrition from mycorrhizal fungi such as
Samuel L Chen et al.
Frontiers in plant science, 10, 1116-1116 (2019-10-15)
Pre-mRNA alternative splicing is a conserved mechanism for eukaryotic cells to leverage existing genetic resources to create a diverse pool of protein products. It is regulated in coordination with other events in RNA metabolism such as transcription, polyadenylation, RNA transport
Bioactivity-Guided Isolation of Neuritogenic Factor from the Seeds of the Gac Plant (Momordica cochinchinensis)
Mazzio E, et al.
Evidence-Based Complementary and Alternative Medicine : ECAM, 2018 (2018)
Xing-Min Geng et al.
Frontiers in plant science, 7, 26-26 (2016-02-10)
Production of reactive oxygen species (ROS) can be accelerated under various biotic and abiotic stresses causing lipid peroxidation, protein degradation, enzyme inactivation, and DNA damage. Superoxide reductase (SOR) is a novel antioxidant enzyme from Pyrococcus furiosus and is employed by
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