推荐产品
储存温度
−20°C
一般描述
植物亲水蛋白抽提试剂盒（Plant Hydrophilic Protein Extraction）是专为植物生命科学应用而设计的产品，能够从任意植物种类或组织中抽提亲水蛋白的定性样品。本试剂盒包含两类试剂：一份植物特异性的蛋白酶抑制剂cocktail、一份用于抽提亲水蛋白质的专用配方试剂（植物蛋白抽提试剂第1类试剂，Plant Protein Extraction Reagent Type 1）。蛋白酶抑制剂cocktail是作用于丝氨酸、半胱氨酸、金属蛋白酶、天冬氨酸与氨肽酶等酶类的多种蛋白酶的广谱抑制剂混合物。本品含有胃酶抑素A，E-64，亮肽素，4-（2-氨基乙基）苯磺酰氟（AEBSF）中，1,10-菲咯啉，和苯丁抑制素等多种成分。经验证，cocktail抑制剂混合物能够在抽提过程中高效避免蛋白降解的发生。
在去除了多酚，鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用一个专用配方试剂重悬，来抽提亲水蛋白。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得水溶性的蛋白样品，用于下游蛋白组学分析。
在去除了多酚，鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用一个专用配方试剂重悬，来抽提亲水蛋白。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得水溶性的蛋白样品，用于下游蛋白组学分析。
数量
足够完成20份 10-250 mg/份植物组织样品的独立抽提制备操作
WGK
WGK 1
闪点(°F)
185.0 °F
闪点(°C)
85 °C
法规信息
新产品
Ramesh Sundar Amalraj et al.
Electrophoresis, 31(12), 1959-1974 (2010-06-22)
Sugarcane is an important commercial crop cultivated for its stalks and sugar is a prized commodity essential in human nutrition. Proteomics of sugarcane is in its infancy, especially when dealing with the stalk tissues, where there is no study to
Yassel Ramos et al.
Analytical biochemistry, 377(2), 134-140 (2008-04-04)
We investigate the selectivity achieved after differential solubilization of bacterial proteomes following two procedures, both based on successive extraction of proteins in solutions of increasing solubilizing power. Recently, these procedures have gained notable popularity and several commercial kits are now
M Cilia et al.
Journal of biomolecular techniques : JBT, 20(4), 201-215 (2009-09-02)
Protein extraction methods can vary widely in reproducibility and in representation of the total proteome, yet there are limited data comparing protein isolation methods. The methodical comparison of protein isolation methods is the first critical step for proteomic studies. To
Angus L Dawe et al.
Methods in molecular biology (Clifton, N.J.), 722, 225-236 (2011-05-19)
The interaction of the filamentous fungal plant pathogen Cryphonectria parasitica with its virulence-attenuating viruses provides a unique platform to explore the molecular biology and genetics of virus-host interactions. Following the development of transformation procedures for this fungus, subsequent advances include
Siew-Yong Koay et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(22), 2179-2183 (2011-06-22)
Orthosiphon aristatus is a traditionally used medicinal plant. In order to study the proteome of the plant, we have developed a simple plant protein extraction method by direct extraction of protein using a modified 2D-gel compatible tris-sucrose buffer followed by
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