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Merck
CN

P9752

Sigma-Aldrich

Phosphoriboisomerase from spinach

Type I, partially purified powder, ≥40 units/mg protein (biuret)

别名:

D-Ribose-5-phosphate ketol-isomerase

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

spinach

质量水平

类型

Type I

表单

partially purified powder

比活

≥40 units/mg protein (biuret)

储存温度

−20°C

应用

Phosphoriboisomerase is used to study the ribulose monophosphate pathway. Phosphoriboisomerase from spinach is used in enzyme assays to convert ribose 5-phosphate to ribulose 5-phosphate.

生化/生理作用

Phosphoriboisomerase is responsible for the converstion of ribose 5-phosphate to ribulose 5-phosphate.

单位定义

One unit will convert 1.0 μmole of D-ribose 5-phosphate to D-ribulose 5-phosphate per min at pH 7.7 at 30 °C.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Mirjam M C Wamelink et al.
Journal of molecular medicine (Berlin, Germany), 88(9), 931-939 (2010-05-26)
Ribose 5-phosphate isomerase (RPI) deficiency is an enzymopathy of the pentose phosphate pathway. It manifests with progressive leukoencephalopathy and peripheral neuropathy and belongs, with one sole diagnosed case, to the rarest human disorders. The single patient was found compound heterozygous
Thomas E Edwards et al.
BMC structural biology, 11, 39-39 (2011-10-15)
Ribose-5-phosphate isomerase is an enzyme that catalyzes the interconversion of ribose-5-phosphate and ribulose-5-phosphate. This family of enzymes naturally occurs in two distinct classes, RpiA and RpiB, which play an important role in the pentose phosphate pathway and nucleotide and co-factor
Z Wu et al.
Journal of applied microbiology, 106(1), 41-48 (2009-01-06)
This paper describes optimization of electrotransformation of Mu transposition complexes into Lactococcus lactis cells and identification of genes affecting nisin production. The highest transformation efficiency, 1.1 x 10(2) transformants microg(-1) of input transposon DNA, was achieved when cells were grown
Richard W Strange et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 65(Pt 12), 1214-1217 (2010-01-08)
Ribose-5-phosphate isomerase is a ubiquitous intracellular enzyme of bacterial, plant and animal origin that is involved in the pentose phosphate cycle, an essential component of cellular carbohydrate metabolism. Specifically, the enzyme catalyses the reversible conversion of ribose 5-phosphate to ribulose
Junho Jung et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 65(Pt 11), 1141-1144 (2009-11-20)
Rare sugars are used for many industrial and medical purposes and are produced by the interconversion between aldoses and ketoses catalyzed by sugar and sugar-phosphate isomerases. Recently, Clostridium thermocellum d-ribose-5-phosphate isomerase (CTRPI), an aldose-ketose isomerase, was cloned in order to

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