一般描述
λ 蛋白磷酸酶是一种在 大肠杆菌中表达的重组蛋白 。它需要 Mn 2 + 或 Ni 2 + 作为活化剂。
生化/生理作用
λ 蛋白磷酸酶已被用于对海拉细胞提取物进行去磷酸化。
可用于从蛋白质中的丝氨酸、苏氨酸或酪氨酸残基释放磷酸基团。对磷酸化组氨酸残基也有活性。
单位定义
在 30℃，pH 7.5的条件下，一单位每分钟水解 1 nmol 对硝基苯磷酸盐。
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Expression, purification, crystallization, and biochemical characterization of a recombinant protein phosphatase.
Zhuo S
The Journal of Biological Chemistry, 268(24), 17754-17761 (1993)
Proliferating cell nuclear antigen-dependent rapid recruitment of Cdt1 and CRL4Cdt2 at DNA-damaged sites after UV irradiation in HeLa cells.
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The Journal of Biological Chemistry, 285(53), 41993-42000 (2010)
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The Biochemical journal, 260(3), 931-934 (1989-06-15)
Infection of Escherichia coli with phage lambda gt10 resulted in the appearance of a protein phosphatase with activity towards 32P-labelled casein. Activity reached a maximum near the point of cell lysis and declined thereafter. The phosphatase was stimulated 30-fold by
Kyoko Chiba et al.
Molecular biology of the cell, 28(26), 3857-3869 (2017-11-03)
In neurons, amyloid β-protein precursor (APP) is transported by binding to kinesin-1, mediated by JNK-interacting protein 1b (JIP1b), which generates the enhanced fast velocity (EFV) and efficient high frequency (EHF) of APP anterograde transport. Previously, we showed that EFV requires
Xiaoyan Yu et al.
The Journal of comparative neurology, 529(3), 481-500 (2020-05-26)
Afferent activity dynamically regulates neuronal properties and connectivity in the central nervous system. The Fragile X mental retardation protein (FMRP) is an RNA-binding protein that regulates cellular and synaptic properties in an activity-dependent manner. Whether and how FMRP level and
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