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表单
ammonium sulfate suspension
比活
5.0-20.0 units/mg protein (biuret)
储存温度
2-8°C
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一般描述
丙酮酸脱羧酶(PDC)通常出现在萌发阶段的植物种子中，特别是当整个植物胚都包裹氧透过性种皮时。
应用
丙酮酸脱羧酶(PDC)可用于研究参与硫胺素焦磷酸(TPP)结合的残基。 也可用于研究植物发酵途径调控。
丙酮酸脱羧酶（来自面包酵母/酿酒酵母）已用于评估有益代谢酶调控系统鉴定(SIMMER,systematic identification of meaningful metabolic enzyme regulation)方法发现未知酵母调控作用的能力。
生化/生理作用
丙酮酸脱羧酶(PDC)是可在细胞质中催化丙酮酸脱羧成为乙醛和二氧化碳的同四聚体酶。 丙酮酸脱羧酶(PDC)依赖辅因子硫胺素焦磷酸(TPP)和镁。 PDC具有1个β-α-β结构，形成平行β-折叠片。
丙酮酸脱羧酶(PDC)活跃参与多种细菌、酵母和植物种子的厌氧代谢。
单位定义
在pH 6.0、25℃条件下，一个单位每分钟可将1.0 μmole丙酮酸转化为乙醛。
外形
溶于3.2 M (NH4)2SO4(pH 6.5)的悬液，添加5%甘油、5 mM磷酸钾、1 mM乙酸镁、0.5 mM EDTA和25 μM辅羧酶帮助稳定。
制备说明
分离纯化处理时不曾使用重金属。
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Bijayalaxmi Mohanty et al.
Annals of botany, 91 Spec No, 291-300 (2003-01-02)
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Systems-level analysis of mechanisms regulating yeast metabolic flux
Hackett S R, et al.
Science, 354(6311), aaf2786-aaf2786 (2016)
Substrate activation behaviour of pyruvate decarboxylase from Pisum sativum cv. Miko
Dietrich A and Konig S
Febs Letters, 400(1), 42-44 (1997)
Clara C Posthuma et al.
Applied and environmental microbiology, 68(2), 831-837 (2002-02-02)
Purification of xylulose 5-phosphate phosphoketolase (XpkA), the central enzyme of the phosphoketolase pathway (PKP) in lactic acid bacteria, and cloning and sequence analysis of the encoding gene, xpkA, from Lactobacillus pentosus MD363 are described. xpkA encodes a 788-amino-acid protein with
Kyung Ok Yu et al.
Applied biochemistry and biotechnology, 166(4), 856-865 (2011-12-14)
The conversion of low-priced glycerol to higher value products has been proposed as a way to improve the economic viability of the biofuels industry. In a previous study, the conversion of glycerol to ethanol in a metabolically engineered strain of
商品
以硫酸铵悬浮液形式提供的酶的使用指南
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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