P9370
Monoclonal Anti-PRMT3 antibody produced in mouse
~2 mg/mL, clone PRMT3-367, purified immunoglobulin, buffered aqueous solution
别名:
Anti-Protein Arginine Methyl Transferase 3
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关于此项目
Conjugate:
unconjugated
Clone:
PRMT3-367, monoclonal
Application:
ARR, ELISA (i), ICC, WB
Citations:
3
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PRMT3-367, monoclonal
form
buffered aqueous solution
mol wt
antigen ~58 kDa
species reactivity
mouse, rat, human
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1-2 μg/mL using whole cell extracts of 293T cell line transfected with human PRMT3
isotype
IgG3
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PRMT3(10196)
General description
Monoclonal Anti-PRMT3 (mouse IgG3 isotype) is derived from the PRMT3-367 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Protein arginine methyltransferase 3 (PRMT3), one of the type I arginine methyltransferases is expressed in heart, small intestine, kidney, ovary, testis, thyroid, and cortex with a cytoplasmic localization within the cells. It consists of two important domains in its N-terminal region, a C2H2 zinc-finger motif and a tyrosine-phosphorylation consensus sequence. This gene is located on human chromosome 11p15.1.
Immunogen
synthetic peptide corresponding to amino acids 501-514 of rat PRMT3 conjugated to KLH.
Application
Monoclonal Anti-PRMT3 antibody produced in mouse may be used in:
- immunoblotting
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Does not cross-react with PRMT1, 2, 4, 5, or 6.
Protein arginine methyltransferase 3 (PRMT3) helps to catalyze the formation of asymmetric dimethylarginine. It plays a key role in ribosomal function and also in controlling protein synthesis. In apolipoprotein E (apoE) knockout mice, PRMT3 activity inhibition decreases hepatic steatosis. PRMT3 also serves as a specific cofactor for liver X receptor (LXR) transcription.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
此项目有
Protein arginine methylation in estrogen signaling and estrogen-related cancers
Teyssier C, et al.
Trends in Endocrinology and Metabolism, 21(3), 181-189 (2010)
Ribosomal protein S2 is a substrate for mammalian PRMT3 (protein arginine methyltransferase 3)
Swiercz R, et al.
The Biochemical Journal, 386(1), 85-91 (2005)
Inhibition of PRMT3 activity reduces hepatic steatosis without altering atherosclerosis susceptibility in apoE knockout mice
Hoekstra M, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1865(6), 1402-1409 (2019)
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Datasheet
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