产品名称
蛋白酶K-琼脂糖 来源于林伯氏白色念球菌, lyophilized powder
biological source
microbial (T.album
T. ALBUM)
form
lyophilized powder
extent of labeling
30-140 units per g solid
technique(s)
DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable
swelling
1 g swells to ~5 mL
storage temp.
−20°C
Quality Level
Application
蛋白酶K-琼脂糖被用于分离蛋白质和核酸、修饰酶和固定酶。蛋白酶K-琼脂糖已被用于研究家犬的肺炎克雷伯菌(肺炎克雷伯菌)和多房棘球绦虫(Echinococcus multilocularis)感染。
Biochem/physiol Actions
通过将蛋白酶K固定在不溶性基质上,如琼脂糖,可将其用于更多应用中。琼脂糖在大多数系统中都是惰性的,并且每单位重量具有很高的活性。利用这种反应性树脂,通过酶与底物在合适介质中的瞬时接触,能够迅速催化酶反应。反应可以在间歇桨液反应器中进行,甚至可以在小柱中进行。无论是哪种情况,不溶性酶都不会污染反应混合物,可以很容易地将其从间歇桨液中滤出或留在色谱柱中。
Features and Benefits
•基质载体上的可回收蛋白酶 K。
•可以利用旋转柱处理或分批处理。
•可以利用旋转柱处理或分批处理。
Other Notes
在pH 7.5、37℃条件下,一单位每分钟可水解尿素变性血红蛋白并产生相当于1.0 μ摩尔 (181 μg) 酪氨酸的显色(使用Folin-Ciocalteu试剂显色)。
Physical form
用乳糖稳定化的冻干粉
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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