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Merck
CN

P9290

蛋白酶K-琼脂糖 来源于林伯氏白色念球菌

lyophilized powder

别名:

蛋白酶K-琼脂糖

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UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
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产品名称

蛋白酶K-琼脂糖 来源于林伯氏白色念球菌, lyophilized powder

biological source

microbial (T.album
T. ALBUM)

form

lyophilized powder

extent of labeling

30-140 units per g solid

technique(s)

DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable

swelling

1 g swells to ~5 mL

storage temp.

−20°C

Quality Level

Application

蛋白酶K-琼脂糖被用于分离蛋白质和核酸、修饰酶和固定酶。蛋白酶K-琼脂糖已被用于研究家犬的肺炎克雷伯菌(肺炎克雷伯菌)和多房棘球绦虫(Echinococcus multilocularis)感染。

Biochem/physiol Actions

通过将蛋白酶K固定在不溶性基质上,如琼脂糖,可将其用于更多应用中。琼脂糖在大多数系统中都是惰性的,并且每单位重量具有很高的活性。利用这种反应性树脂,通过酶与底物在合适介质中的瞬时接触,能够迅速催化酶反应。反应可以在间歇桨液反应器中进行,甚至可以在小柱中进行。无论是哪种情况,不溶性酶都不会污染反应混合物,可以很容易地将其从间歇桨液中滤出或留在色谱柱中。

Features and Benefits

•基质载体上的可回收蛋白酶 K。
•可以利用旋转柱处理或分批处理。

Other Notes

在pH 7.5、37℃条件下,一单位每分钟可水解尿素变性血红蛋白并产生相当于1.0 μ摩尔 (181 μg) 酪氨酸的显色(使用Folin-Ciocalteu试剂显色)。

Physical form

用乳糖稳定化的冻干粉

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
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分析证书(COA)

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Shai Eyal et al.
Development (Cambridge, England), 146(4) (2019-02-13)
Sesamoid bones are small auxiliary bones that form near joints and contribute to their stability and function. Thus far, providing a comprehensive developmental model or classification system for this highly diverse group of bones has been challenging. Here, we compare
P Champeil et al.
The Journal of biological chemistry, 273(12), 6619-6631 (1998-04-18)
Treatment of rabbit sarcoplasmic reticulum Ca2+-ATPase with a variety of proteases, including elastase, proteinase K, and endoproteinases Asp-N and Glu-C, results in accumulation of soluble fragments starting close to the ATPase phosphorylation site Asp351 and ending in the Lys605-Arg615 region
Hirokazu Kouguchi et al.
Experimental parasitology, 128(1), 50-56 (2011-02-09)
We show that a conventionally purified glycoprotein component of Echinococcus multilocularis protoscolex, designated as Emgp-89, may be useful as a serodiagnostic antigen for detecting E. multilocularis infection in dogs domesticated in endemic areas. Emgp-89 was obtained from the parasite material
Yi Zuo et al.
PloS one, 14(10), e0223299-e0223299 (2019-10-04)
Oral herpes is a highly prevalent infection caused by herpes simplex virus 1 (HSV-1). After an initial infection of the oral cavity, HSV-1 remains latent in sensory neurons of the trigeminal ganglia. Episodic reactivation of the virus leads to the
David da Silva Barreira et al.
Scientific reports, 13(1), 1163-1163 (2023-01-21)
Biofilms represent a major concern in the food industry and healthcare. The use of probiotic bacteria and their derivatives as an alternative to conventional treatments to fight biofilm development is a promising option that has provided convincing results in the

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