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Merck
CN

P9290

蛋白酶K-琼脂糖 来源于林伯氏白色念球菌

lyophilized powder

别名:

蛋白酶K-琼脂糖

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关于此项目

UNSPSC Code:
41106500
NACRES:
NA.56
MDL number:
Form:
lyophilized powder
Biological source:
microbial (T.album
T. ALBUM)
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biological source

microbial (T.album
T. ALBUM)

form

lyophilized powder

extent of labeling

30-140 units per g solid

technique(s)

DNA extraction: suitable, RNA extraction: suitable, protein extraction: suitable

swelling

1 g swells to ~5 mL

storage temp.

−20°C

Quality Level

Application

蛋白酶K-琼脂糖被用于分离蛋白质和核酸、修饰酶和固定酶。蛋白酶K-琼脂糖已被用于研究家犬的肺炎克雷伯菌(肺炎克雷伯菌)和多房棘球绦虫(Echinococcus multilocularis)感染。

Biochem/physiol Actions

通过将蛋白酶K固定在不溶性基质上,如琼脂糖,可将其用于更多应用中。琼脂糖在大多数系统中都是惰性的,并且每单位重量具有很高的活性。利用这种反应性树脂,通过酶与底物在合适介质中的瞬时接触,能够迅速催化酶反应。反应可以在间歇桨液反应器中进行,甚至可以在小柱中进行。无论是哪种情况,不溶性酶都不会污染反应混合物,可以很容易地将其从间歇桨液中滤出或留在色谱柱中。

Features and Benefits

•基质载体上的可回收蛋白酶 K。
•可以利用旋转柱处理或分批处理。

Physical form

用乳糖稳定化的冻干粉

Other Notes

在pH 7.5、37℃条件下,一单位每分钟可水解尿素变性血红蛋白并产生相当于1.0 μ摩尔 (181 μg) 酪氨酸的显色(使用Folin-Ciocalteu试剂显色)。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Zohar Erez et al.
Nature, 541(7638), 488-493 (2017-01-19)
Temperate viruses can become dormant in their host cells, a process called lysogeny. In every infection, such viruses decide between the lytic and the lysogenic cycles, that is, whether to replicate and lyse their host or to lysogenize and keep
David da Silva Barreira et al.
Scientific reports, 13(1), 1163-1163 (2023-01-21)
Biofilms represent a major concern in the food industry and healthcare. The use of probiotic bacteria and their derivatives as an alternative to conventional treatments to fight biofilm development is a promising option that has provided convincing results in the
M J Pearse et al.
Immunology letters, 18(3), 219-223 (1988-07-01)
This report describes a method for preparing both DNA and RNA simultaneously from as few as 5 X 10(5) lymphoid cells. The method is suitable for cultured cells or any tissue from which a cell suspension can be prepared and
Guillaume Saubeau et al.
Molecules (Basel, Switzerland), 19(10), 15374-15390 (2014-09-30)
The induction of plant immunity by Pathogen Associated Molecular Patterns (PAMPs) constitutes a powerful strategy for crop protection. PAMPs indeed induce general defense responses in plants and thus increase plant resistance to pathogens. Phytophthora infestans culture filtrates (CCFs) are known
Yuan Gao et al.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi, 31(7), 786-790 (2010-12-18)
To type Klebsiella pneumonia through methods including pulse-field gel electrophoresis (PFGE) in combination with multilocus sequence typing. Four selected different EPs, referring to the Standard Operating Procedure of PulseNet China, were used. The single colony of Klebsiella pneumonia was quantified

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