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生物来源
microbial (T.album
T. ALBUM)
质量水平
形式
lyophilized powder
标记范围
30-140 units per g solid
技术
DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable
溶胀
1 g swells to ~5 mL
储存温度
−20°C
应用
蛋白酶K-琼脂糖被用于分离蛋白质和核酸、修饰酶和固定酶。蛋白酶K-琼脂糖已被用于研究家犬的肺炎克雷伯菌（肺炎克雷伯菌）和多房棘球绦虫（Echinococcus multilocularis）感染。
生化/生理作用
通过将蛋白酶K固定在不溶性基质上，如琼脂糖，可将其用于更多应用中。琼脂糖在大多数系统中都是惰性的，并且每单位重量具有很高的活性。利用这种反应性树脂，通过酶与底物在合适介质中的瞬时接触，能够迅速催化酶反应。反应可以在间歇桨液反应器中进行，甚至可以在小柱中进行。无论是哪种情况，不溶性酶都不会污染反应混合物，可以很容易地将其从间歇桨液中滤出或留在色谱柱中。
特点和优势
•基质载体上的可回收蛋白酶 K。
•可以利用旋转柱处理或分批处理。
•可以利用旋转柱处理或分批处理。
单位定义
在pH 7.5、37℃条件下，一单位每分钟可水解尿素变性血红蛋白并产生相当于1.0 μ摩尔 (181 μg) 酪氨酸的显色（使用Folin-Ciocalteu试剂显色）。
外形
用乳糖稳定化的冻干粉
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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