特异性
一种具有包括细胞外丝氨酸蛋白酶在内至少三种蛋白水解活性的混合物。通常,丝氨酸蛋白酶可显示出广泛的底物特异性,而这被视为通过分子中由一个Asp、一个His和Ser残基组成的活性位点所介导的。该酶更倾向于水解谷氨酸或天冬氨酸的羧基侧的肽键。
应用
蛋白酶通常用于核酸的分离过程,通过补充0.2%十二烷基硫酸钠和10 mM EDTA并孵育0.5-3.0小时。
该酶比已知碱性蛋白酶在更高的pH范围内更具活性,即使在0.2N NaOH溶液中也显示出了蛋白水解活性。该酶用于不溶性蛋白的蛋白水解和蛋白的结构研究。
其他说明
一种不常见的非特异性蛋白酶。
质量
含有约 25% 的醋酸钙。
物理属性
通过在80 °C以上加热15-20分钟以完全灭活。
单位定义
在 pH7.5,37℃ 下,一个单元每分钟将水解酪蛋白,并产生相当于 1.0 μmole(181 μg)酪氨酸的颜色(使用 Folin-Ciocalteu 试剂显色)。
制备说明
收集自灰色链霉菌的培养基肉汤中。
警示用语:
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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